LOCUS       V014753                10074 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014753
VERSION     V014753
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3547..4788
                     /gene="IDH1"
                     /label="Isocitrate dehydrogenase [NADP] cytoplasmic"
                     /note="Isocitrate dehydrogenase [NADP] cytoplasmic from
                     Homo sapiens. Accession#: O75874"
     CDS             4789..4812
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    4824..5410
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5430..6026
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6043..6631
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6838..7471
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7647..7677)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7692..7713)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8001..8589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8763..9620)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9621..9725)
                     /label="AmpR promoter"
     polyA_signal    9773..9907
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccaccatgt ccaaaaaaat cagtggcggt tctgtggtag agatgcaagg agatgaaatg
     3601 acacgaatca tttgggaatt gattaaagag aaactcattt ttccctacgt ggaattggat
     3661 ctacatagct atgatttagg catagagaat cgtgatgcca ccaacgacca agtcaccaag
     3721 gatgctgcag aagctataaa gaagcataat gttggcgtca aatgtgccac tatcactcct
     3781 gatgagaaga gggttgagga gttcaagttg aaacaaatgt ggaaatcacc aaatggcacc
     3841 atacgaaata ttctgggtgg cacggtcttc agagaagcca ttatctgcaa aaatatcccc
     3901 cggcttgtga gtggatgggt aaaacctatc atcataggtc atcatgctta tggggatcaa
     3961 tacagagcaa ctgattttgt tgttcctggg cctggaaaag tagagataac ctacacacca
     4021 agtgacggaa cccaaaaggt gacatacctg gtacataact ttgaagaagg tggtggtgtt
     4081 gccatgggga tgtataatca agataagtca attgaagatt ttgcacacag ttccttccaa
     4141 atggctctgt ctaagggttg gcctttgtat ctgagcacca aaaacactat tctgaagaaa
     4201 tatgatgggc gttttaaaga catctttcag gagatatatg acaagcagta caagtcccag
     4261 tttgaagctc aaaagatctg gtatgagcat aggctcatcg acgacatggt ggcccaagct
     4321 atgaaatcag agggaggctt catctgggcc tgtaaaaact atgatggtga cgtgcagtcg
     4381 gactctgtgg cccaagggta tggctctctc ggcatgatga ccagcgtgct ggtttgtcca
     4441 gatggcaaga cagtagaagc agaggctgcc cacgggactg taacccgtca ctaccgcatg
     4501 taccagaaag gacaggagac gtccaccaat cccattgctt ccatttttgc ctggaccaga
     4561 gggttagccc acagagcaaa gcttgataac aataaagagc ttgccttctt tgcaaatgct
     4621 ttggaagaag tctctattga gacaattgag gctggcttca tgaccaagga cttggctgct
     4681 tgcattaaag gtttacccaa tgtgcaacgt tctgactact tgaatacatt tgagttcatg
     4741 gataaacttg gagaaaactt gaagatcaaa ctagctcagg ccaaacttga ctacaaggac
     4801 gacgatgaca agtgaggatc ccgcccctct ccctcccccc cccctaacgt tactggccga
     4861 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4921 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4981 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5041 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5101 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5161 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5221 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5281 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5341 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     5401 tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     5461 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5521 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     5581 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5641 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5701 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5761 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     5821 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     5881 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     5941 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6001 ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga acaatcaacc tctggattac
     6061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6421 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6601 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     6661 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6721 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6781 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     6841 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6901 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     6961 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7021 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7081 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7141 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7201 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7261 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7321 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7381 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7441 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7501 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     7561 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7741 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7801 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7861 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7921 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7981 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8041 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8101 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8161 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8221 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8281 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8341 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8401 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8461 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8521 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8581 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8641 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8701 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8761 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8821 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8881 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8941 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9001 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9061 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9121 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9181 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9241 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9301 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9361 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9421 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9481 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9541 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9601 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9661 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9721 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     9781 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9841 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9901 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9961 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10021 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
