LOCUS       V010440                10080 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010440
VERSION     V010440
KEYWORDS    pLVX-EF1alpha-Tet3G.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2388..3569
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3670..4413
                     /label="Tet-On(R) 3G"
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     misc_feature    4425..5010
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5011..5802
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    6002..6590
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6798..7431
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7608..7638)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7653..7674)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7962..8547)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8721..9578)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9579..9683)
                     /label="AmpR promoter"
     polyA_signal    9731..9865
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgctcgagga gcttggccca ttgcatacgt tgtatccata
     2221 tcataatatg tacagagtaa ttcatacaaa aggactcgcc cctgccttgg ggaatcccag
     2281 ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc ccgccccctc
     2341 acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct ccggtgcccg
     2401 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2461 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2521 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2581 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2641 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacgcccct
     2701 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     2761 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cttgggcgct
     2821 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     2881 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     2941 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     3001 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     3061 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     3121 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3181 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     3241 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     3301 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     3361 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3421 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     3481 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     3541 gtttttttct tccatttcag gtgtcgtgag agctcgttta gtgaaccgtc agatcgcctg
     3601 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccaaattctc
     3661 gaggccacca tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc
     3721 aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     3781 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     3841 gagatgctgg acaggcatca tacccactcc tgccccctgg aaggcgagtc atggcaagac
     3901 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     3961 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     4021 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     4081 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     4141 agagagacac ctaccaccga ttctatgccc ccacttctga aacaagcaat tgagctgttc
     4201 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     4261 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     4321 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     4381 cttgacgatt ttgaccttga catgctcccc gggtaaggat cccgcccctc tccctccccc
     4441 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     4501 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     4561 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4621 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4681 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4741 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4801 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4861 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4921 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4981 gaaaaacacg atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt
     5041 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5101 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5161 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5221 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5281 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5341 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5401 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5461 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5521 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5581 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5641 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5701 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5761 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga
     5821 gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca
     5881 tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc
     5941 tgcgcgacgg cgagctgctc accacccact cgagtctaga gggccccgac gcgtctggaa
     6001 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6361 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     6601 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6661 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt ttccagtcac
     6721 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6781 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     6841 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     6901 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     6961 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     7021 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     7081 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     7141 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     7201 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7261 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7321 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7381 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7441 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7501 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg
     7561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag
     8521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9721 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9841 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9901 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
     9961 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10021 caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
