LOCUS       V012693                 8753 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012693
VERSION     V012693
KEYWORDS    pLVX-DsRed-Monomer-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2815..3489
                     /label="DsRed-Monomer"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
     misc_feature    3490..3555
                     /label="MCS"
                     /note="multiple cloning site"
     promoter        3588..4087
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4108..4704
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4721..5309
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5516..6149
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6278..6294)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6302..6318)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6326..6356)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6371..6392)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6680..7268)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7442..8299)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8300..8404)
                     /label="AmpR promoter"
     polyA_signal    8452..8586
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggtcgc caccatggac
     2821 aacaccgagg acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga gggctccgtg
     2881 aacggccact acttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag
     2941 accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     3001 ccccagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3061 atgaagctgt ccttccccga gggcttcacc tgggagcgct ccatgaactt cgaggacggc
     3121 ggcgtggtgg aggtgcagca ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg
     3181 aagttcaagg gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa gactgccggc
     3241 tgggagccct ccaccgagaa gctgtacccc caggacggcg tgctgaaggg cgagatctcc
     3301 cacgccctga agctgaagga cggcggccac tacacctgcg acttcaagac cgtgtacaag
     3361 gccaagaagc ccgtgcagct gcccggcaac cactacgtgg actccaagct ggacatcacc
     3421 aaccacaacg aggactacac cgtggtggag cagtacgagc acgccgaggc ccgccactcc
     3481 ggctcccagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     3541 gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg taggggaggc
     3601 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     3661 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     3721 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     3781 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     3841 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     3901 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     3961 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4021 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     4081 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4141 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4201 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4261 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4321 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4381 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4441 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4501 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4561 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4621 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4681 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
     4741 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4801 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4861 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4921 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4981 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5041 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5101 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     5161 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5221 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5281 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     5341 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5401 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     5461 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     5521 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     5581 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     5641 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     5701 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     5761 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     5821 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     5881 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     5941 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     6001 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6061 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6121 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6181 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     6241 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8401 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
     8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8581 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     8641 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     8701 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
