LOCUS       V013574                10287 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013574
VERSION     V013574
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2807..3514
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             3548..5080
                     /label="VSV-G"
                     /note="vesicular stomatitis virus G glycoprotein"
     promoter        5122..5621
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5642..6238
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6255..6843
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7050..7683
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7860..7890)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7905..7926)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8214..8802)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8976..9833)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9834..9938)
                     /label="AmpR promoter"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg
     2821 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2881 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2941 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3001 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3061 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3121 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3181 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3241 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3301 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3361 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3421 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3481 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gaggcggcgg
     3541 agggagtatg aagtgccttt tgtacttagc ctttttattc attggggtga attgcaagtt
     3601 caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt ctaattacca
     3661 ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca cagccttaca
     3721 agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt gtcatgcttc
     3781 caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa cacattccat
     3841 ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa cgaaacaagg
     3901 aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg tgacggatgc
     3961 cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat acacaggaga
     4021 atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc ccactgtcca
     4081 taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt ctaacctcat
     4141 ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg gaaaggaggg
     4201 cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct gcaaaatgca
     4261 atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga tggctgataa
     4321 ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta tctctgctcc
     4381 atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct tggattattc
     4441 cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc cagtggatct
     4501 cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa tcaatggtac
     4561 cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa tcctctcaag
     4621 aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg actgggcacc
     4681 atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag gatataagtt
     4741 tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta gctcaaaggc
     4801 tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg atgatgagag
     4861 tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag aaggttggtt
     4921 cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa tcattggact
     4981 attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca ccaagaaaag
     5041 acagatttat acagacatag agatgaaccg acttggaaag taaggatcca ccggatctag
     5101 ataactgatc ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     5161 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     5221 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     5281 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     5341 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     5401 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     5461 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     5521 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5581 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     5641 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5701 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5761 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5821 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5881 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5941 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6001 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6061 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6121 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6181 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6241 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6301 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6361 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6421 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6481 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6541 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6601 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6661 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6721 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6781 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6841 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     6901 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6961 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7021 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     7081 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     7141 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     7201 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     7261 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     7321 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     7381 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     7441 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     7501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7681 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7741 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg
     7801 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7861 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7981 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8041 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8101 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8161 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8221 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8281 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8341 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8401 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8461 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8521 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8581 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8641 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8701 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8761 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8821 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8881 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8941 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9001 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9061 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9121 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9181 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9241 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9301 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9361 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9421 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9481 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9541 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9601 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9661 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9721 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9781 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9961 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10081 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10141 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10201 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10261 ttaaatatgt actacaaact tagtagt
//
