LOCUS       V014053                10160 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014053
VERSION     V014053
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2826..4886
                     /gene="TGM2"
                     /label="Protein-glutamine gamma-glutamyltransferase 2"
                     /note="Protein-glutamine gamma-glutamyltransferase 2 from
                     Homo sapiens. Accession#: P21980"
     CDS             4893..4916
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        4948..5447
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5468..6064
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6081..6669
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6876..7509
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7686..7716)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7731..7752)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8040..8628)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8802..9659)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9660..9764)
                     /label="AmpR promoter"
     polyA_signal    9812..9946
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc cgaggagctg gtcttagaga ggtgtgatct ggagctggag accaatggcc
     2881 gagaccacca cacggccgac ctgtgccggg agaagctggt ggtgcgacgg ggccagccct
     2941 tctggctgac cctgcacttt gagggccgca actacgaggc cagtgtagac agtctcacct
     3001 tcagtgtcgt gaccggccca gcccctagcc aggaggccgg gaccaaggcc cgttttccac
     3061 taagagatgc tgtggaggag ggtgactgga cagccaccgt ggtggaccag caagactgca
     3121 ccctctcgct gcagctcacc accccggcca acgcccccat cggcctgtat cgcctcagcc
     3181 tggaggcctc cactggctac cagggatcca gctttgtgct gggccacttc attttgctct
     3241 tcaacgcctg gtgcccagcg gatgctgtgt acctggactc ggaagaggag cggcaggagt
     3301 atgtcctcac ccagcagggc tttatctacc agggctcggc caagttcatc aagaacatac
     3361 cttggaattt tgggcagttt gaagatggga tcctagacat ctgcctgatc cttctagatg
     3421 tcaaccccaa gttcctgaag aacgccggcc gtgactgctc ccgccgcagc agccccgtct
     3481 acgtgggccg ggtggtgagt ggcatggtca actgcaacga tgaccagggt gtgctgctgg
     3541 gacgctggga caacaactac ggggacggcg tcagccccat gtcctggatc ggcagcgtgg
     3601 acatcctgcg gcgctggaag aaccacggct gccagcgcgt caagtatggc cagtgctggg
     3661 tcttcgccgc cgtggcctgc acagtgctga ggtgcctggg catccctacc cgcgtcgtga
     3721 ccaactacaa ctcggcccat gaccagaaca gcaaccttct catcgagtac ttccgcaatg
     3781 agtttgggga gatccagggt gacaagagcg agatgatctg gaacttccac tgctgggtgg
     3841 agtcgtggat gaccaggccg gacctgcagc cggggtacga gggctggcag gccctggacc
     3901 cagcgcccca ggagaagagc gaagggacgt actgctgtgg cccagttcca gttcgtgcca
     3961 tcaaggaggg cgacctgagc accaagtacg atgcgccctt tgtctttgcg gaggtcaatg
     4021 ccgacgtggt agactggatc cagcaggacg atgggtctgt gcacaaatcc atcaaccgtt
     4081 ccctgatcgt tgggctgaag atcagcacta agagcgtggg ccgagacgag cgggaggata
     4141 tcacccacac ctacaaatac ccagaggggt cctcagagga gagggaggcc ttcacaaggg
     4201 cgaaccacct gaacaaactg gccgagaagg aggagacagg gatggccatg cggatccgtg
     4261 tgggccagag catgaacatg ggcagtgact ttgacgtctt tgcccacatc accaacaaca
     4321 ccgctgagga gtacgtctgc cgcctcctgc tctgtgcccg caccgtcagc tacaatggga
     4381 tcttggggcc cgagtgtggc accaagtacc tgctcaacct caacctggag cctttctctg
     4441 agaagagcgt tcctctttgc atcctctatg agaaataccg tgactgcctt acggagtcca
     4501 acctcatcaa ggtgcgggcc ctcctcgtgg agccagttat caacagctac ctgctggctg
     4561 agagggacct ctacctggag aatccagaaa tcaagatccg gatccttggg gagcccaagc
     4621 agaaacgcaa gctggtggct gaggtgtccc tgcagaaccc gctccctgtg gccctggaag
     4681 gctgcacctt cactgtggag ggggccggcc tgactgagga gcagaagacg gtggagatcc
     4741 cagaccccgt ggaggcaggg gaggaagtta aggtgagaat ggacctgctg ccgctccaca
     4801 tgggcctcca caagctggtg gtgaacttcg agagcgacaa gctgaaggct gtgaagggct
     4861 tccggaatgt catcattggc cccgccgaat tcgattacaa ggatgacgac gataagtgag
     4921 gatcccgcga ctctagataa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4981 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     5041 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     5101 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     5161 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     5221 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     5281 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     5341 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     5401 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     5461 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5521 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5581 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5641 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5701 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5761 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5821 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5881 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5941 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6001 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6061 tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6121 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6181 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6241 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6301 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6361 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6421 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6481 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6541 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6601 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6661 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     6721 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6781 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6841 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     6901 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     6961 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7021 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7081 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7141 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7201 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7261 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7321 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7501 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7561 gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag
     7621 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7681 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7741 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7801 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8461 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9781 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     9841 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9901 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9961 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10021 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10081 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
    10141 tgtactacaa acttagtagt
//
