LOCUS       V013649                 9922 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013649
VERSION     V013649
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2835..4484
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    4496..5082
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5083..5874
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    5891..6479
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6686..7319
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7495..7525)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7540..7561)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7849..8437)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8611..9468)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9469..9573)
                     /label="AmpR promoter"
     polyA_signal    9621..9755
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 tctagagcgg ccgcatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     2881 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     2941 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     3001 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     3061 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     3121 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     3181 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     3241 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     3301 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     3361 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     3421 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     3481 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     3541 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     3601 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     3661 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     3721 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     3781 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     3841 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     3901 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     3961 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     4021 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     4081 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     4141 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     4201 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     4261 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     4321 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     4381 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     4441 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaagga tcccgcccct
     4501 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4561 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4621 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4681 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4741 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4801 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4861 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4921 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4981 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5041 tggttttcct ttgaaaaaca cgatgataag cttgccacaa ccatggctga acaagatgga
     5101 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5161 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5221 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     5281 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5341 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5401 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5461 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5521 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5581 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5641 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5701 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5761 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5821 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaacg
     5881 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5941 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6001 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6061 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6121 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6181 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6241 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6301 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6361 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6421 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6481 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     6541 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6601 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6661 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     6721 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     6781 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6841 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     6901 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     6961 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     7021 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     7081 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     7141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7321 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7381 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     7441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7501 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7561 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7681 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7741 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8461 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8521 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8581 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8641 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8701 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8761 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8821 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8881 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8941 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9001 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9061 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9121 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9181 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9241 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9301 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9361 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9421 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9481 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9541 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9601 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     9661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9721 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     9781 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     9841 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     9901 tatgtactac aaacttagta gt
//
