LOCUS       .                       9925 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        1..304
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             983..1699
                     /label="Citrine"
                     /note="enhanced variant of YFP (Heikal et al., 2001)"
     misc_feature    2442..3028
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3039..3059
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             3165..3677
                     /label="Nluc"
                     /note="NanoLuc® luciferase"
     misc_feature    3694..4282
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4489..5122
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5298..5328)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5343..5364)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5652..6240)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6414..7271)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7272..7376)
                     /label="AmpR promoter"
     polyA_signal    7424..7558
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             7726..8359
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8406..8531
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    9028..9261
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             9446..9490
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             9639..9680
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    9752..9869
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
      601 gtgaattcct cgagactagt tctagagcgg ccgcggatcc atgggatgca ccgttagcgc
      661 cgaagacaaa gcagcggcgg agcgaagcaa gatgattgac aaaaatttga gagaggatgg
      721 cgagaaggcg gctcgcgagg tcaaactgtt gcttctgggt gccggagaga gcggcaagag
      781 cactattgta aaacagatga agatcattca tgaggatggc tactcagagg aggaatgtcg
      841 gcaataccgc gcagttgtgt atagtaatac tattcaatcc attatggcaa ttgtaaaggc
      901 gatgggcaac ctccagatcg actttgctga cccgcagagg gcagacgacg cgaggcagct
      961 gttcgccttg agctgcgccg ctatggtgag caagggcgag gagctgttca ccggggtggt
     1021 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1081 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1141 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc
     1201 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1261 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1321 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1381 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1441 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1501 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1561 cgtgttgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1621 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1681 catggacgag ctgtacaaga gcggcgagga acaagggatg ttgccggaag atctgtcagg
     1741 agtgattcgg cggctgtggg ccgaccatgg agtgcaagcg tgctttggga ggagccggga
     1801 ataccaactg aacgattccg ccgcttatta cttgaacgac cttgagcgca ttgctcaaag
     1861 cgattatatt ccaactcaac aagatgtact gagaacacgc gtgaagacaa caggaatagt
     1921 agagactcat ttcacgttta aggatctcca ctttaaaatg ttcgatgttg gaggacaacg
     1981 atctgagcgc aagaagtgga tacactgttt tgagggcgtt accgcgatca ttttctgtgt
     2041 tgctttgtct gcatacgacc tcgtcttggc agaggatgaa gaaatgaatc gaatgcacga
     2101 atccatgaag ttgtttgatt ctatatgcaa caataagtgg tttactgata ccagcatcat
     2161 acttttcttg aataaaaaag acttgtttga ggagaagata acacagagcc cacttacgat
     2221 ttgtttcccg gagtatacgg gagcgaacaa gtatgatgaa gccgcaagtt atatccaatc
     2281 caagtttgag gacctgaata agagaaaaga cacaaaggag atttacacac acttcacctg
     2341 tgccactgac actaaaaacg tccaatttgt tttcgacgcc gttaccgatg tcataataaa
     2401 aaataacctc aaagactgtg gtctttttta agcggccgcc gcccctctcc ctcccccccc
     2461 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     2521 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2581 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2641 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     2701 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     2761 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     2821 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     2881 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     2941 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     3001 aaaacacgat gataagcttg ccacaacccg taccaaagat ggggagtagc aagagcaaga
     3061 cgtcgaattc cggaatcaag cttggtggat cttctcgagg ttactaccat ggtatttggg
     3121 tgggtgaaga aggtcgactt tctcgaggtg gcaccggtgg atccatggtc ttcacactcg
     3181 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     3241 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     3301 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     3361 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     3421 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     3481 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     3541 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     3601 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     3661 gcgaacgcat tctggcgtaa acgcgtctgg aacaatcaac ctctggatta caaaatttgt
     3721 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3781 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3841 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3901 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3961 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4021 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4081 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4141 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4201 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4261 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     4321 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     4381 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     4441 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     4501 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     4561 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     4621 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     4681 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     4741 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     4801 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     4861 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     4921 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4981 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5041 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5101 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5161 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     5221 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5281 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7381 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     7441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7501 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     7561 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     7621 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     7681 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     7741 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     7801 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     7861 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     7921 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     7981 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     8041 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     8101 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     8161 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8221 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8281 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8341 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8401 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8461 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8521 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8581 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8641 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8701 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8761 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8821 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     8881 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8941 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     9001 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     9061 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     9121 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     9181 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9241 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     9301 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9361 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9421 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9481 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9541 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9601 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9661 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9721 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     9781 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9841 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     9901 tttatcgata agcttgggag ttccg
//
