LOCUS       V015192                 9101 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015192
VERSION     V015192
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2821..3720
                     /gene="CD38"
                     /label="ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1"
                     /note="ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
                     from Homo sapiens. Accession#: P28907"
     misc_feature    3740..4319
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4314..5006
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    5023..5611
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5818..6451
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6579..6595)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6603..6619)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6627..6657)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6672..6693)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6981..7569)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7743..8600)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8601..8705)
                     /label="AmpR promoter"
     polyA_signal    8753..8887
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc
     2821 atggccaact gcgagttcag cccggtgtcc ggggacaaac cctgctgccg gctctctagg
     2881 agagcccaac tctgtcttgg cgtcagtatc ctggtcctga tcctcgtcgt ggtgctcgcg
     2941 gtggtcgtcc cgaggtggcg ccagcagtgg agcggtccgg gcaccaccaa gcgctttccc
     3001 gagaccgtcc tggcgcgatg cgtcaagtac actgaaattc atcctgagat gagacatgta
     3061 gactgccaaa gtgtatggga tgctttcaag ggtgcattta tttcaaaaca tccttgcaac
     3121 attactgaag aagactatca gccactaatg aagttgggaa ctcagaccgt accttgcaac
     3181 aagattcttc tttggagcag aataaaagat ctggcccatc agttcacaca ggtccagcgg
     3241 gacatgttca ccctggagga cacgctgcta ggctaccttg ctgatgacct cacatggtgt
     3301 ggtgaattca acacttccaa aataaactat caatcttgcc cagactggag aaaggactgc
     3361 agcaacaacc ctgtttcagt attctggaaa acggtttccc gcaggtttgc agaagctgcc
     3421 tgtgatgtgg tccatgtgat gctcaatgga tcccgcagta aaatctttga caaaaacagc
     3481 acttttggga gtgtggaagt ccataatttg caaccagaga aggttcagac actagaggcc
     3541 tgggtgatac atggtggaag agaagattcc agagacttat gccaggatcc caccataaaa
     3601 gagctggaat cgattataag caaaaggaat attcaatttt cctgcaagaa tatctacaga
     3661 cctgacaagt ttcttcagtg tgtgaaaaat cctgaggatt catcttgcac atctgagatc
     3721 tgagcggccg cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     3781 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     3841 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     3901 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     3961 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4021 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     4081 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctca
     4141 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     4201 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct
     4261 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggccc
     4321 agtccaagca cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg
     4381 acggccacaa gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg
     4441 ccatcaacct gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg
     4501 ccgccttcat gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact
     4561 tcaagaactc ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg
     4621 ccgtgtgcat ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg
     4681 agtccaagtt ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg
     4741 acaactggga gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg
     4801 gcgacgtgag catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca
     4861 ccgtgtacaa ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca
     4921 agctgacccg cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg
     4981 ccatcgcctc cggctccgcc ttgccctgaa cgcgtctgga acaatcaacc tctggattac
     5041 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5101 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5161 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5221 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5281 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5341 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5401 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5461 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5521 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5581 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     5641 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5701 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     5821 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     5881 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     5941 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     6001 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6061 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     6121 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     6181 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     6241 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6421 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6481 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     6541 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6601 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6661 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6721 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6781 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7141 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7381 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7741 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7801 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7861 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7921 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7981 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8041 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8101 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8161 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8221 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8281 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8341 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8401 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8461 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8521 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8581 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8641 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8701 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     8761 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8821 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8881 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     8941 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     9001 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa
     9061 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
