LOCUS       .                      11284 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     intron          2860..3877
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     misc_feature    5270..5856
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5858..6574
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    7206..7794
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8001..8634
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8810..8840)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8855..8876)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9164..9752)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9926..10783)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(10784..10888)
                     /label="AmpR promoter"
     polyA_signal    10936..11070
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg gacattgatt attgactagt
     2221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2461 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2521 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2581 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     2641 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     2701 gggccccccc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt
     2761 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     2821 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt
     2881 ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     2941 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3001 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3061 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     3121 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     3181 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     3241 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     3301 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     3361 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     3421 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     3481 ggctcggggg aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     3541 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     3601 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     3661 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     3721 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     3781 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     3841 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     3901 tgtgctgtct catcattttg gcaaagaatt cacttcaaaa ctgtggctct gcaggaccaa
     3961 actatgtccc ctcccaactc catgtccccc accaccaacg gcaacggtgt ggctatgaat
     4021 ggggccaaga agcagctgga ttttgacccc tccgctgccc cccccttcaa gattgccgac
     4081 atccgcgctg ccatcccccc ccactgctgg gtgaagaacc cctggcgctc cctgtcctac
     4141 gtcctgagag acctgctggt catcctgtcc ttcgccgtgg cggctgccaa gctggactcc
     4201 tggactttct ggcccctgta ctgggtggct cagggcacca tgttctgggc cgtctttgtg
     4261 ctgggccacg actgtggcca tggctccttc tccgacatct ggctgctgaa caacgtgatg
     4321 ggccacatcc tgcactcctc catcctggtg ccctaccatg gctggagaat ctcccacaag
     4381 acccaccacc agaaccacgg caatgtggag aaggatgagt cctgggtgcc tctgcccgag
     4441 aaggtgtaca agtccctgga cacctccact aagttcatgc gcttcaccat ccctctcccc
     4501 atgtttgctt atcctatcta cctgtggacc cgttcccccg gcaagaaggg ctcccacttc
     4561 aacccctact ccgacctgtt cgcccccaac gagcgcgcag cggtcctgat ctccaccctg
     4621 tgctggaccg ccatggccct gctcctgtgc tactcctcct tcatctacgg cttcgctccc
     4681 gtcctgaaga tctacggcgt gccttatctg atcttcgtgg cctggctgga catggtgacc
     4741 tacctgcacc accacggcta cgagcagaag ctgccctggt acagaggcaa agagtggtcc
     4801 tacctgcgcg gcggcctgac caccgtggac cgcgattacg gcgtcatcaa caacatccac
     4861 cacgacattg gcacccacgt catccaccac ctcttccctc agatgcccca ctatcacctg
     4921 gtggaggcca ctcaggcagc caagcacgtg ctgggcaagt actacagaga gcccaagaaa
     4981 tccgggcctt tccccttcca cctgtttggc tacctggtgc gctccctggg cgaggatcac
     5041 tacgtgagcg acaccggcga cgtcgtgttc tatcagtctg acccccacat tcccaagttc
     5101 cgcaccagct ccgccaccac caagtccaag tccagctgat gatatttggc tctgatatat
     5161 gcaggctgtt tatcttgtcc tttgttcgtt tctttctccc agaaacaaat tctctgtttc
     5221 tatgtttctc tgtctctccc gcccccagct ttctttctga gggatcccgc ccctctccct
     5281 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5341 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5401 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5461 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5521 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5581 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5641 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5701 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5761 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5821 tcctttgaaa aacacgatga taagcttgcc acaacccatg gtgagcaagg gcgaggagct
     5881 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5941 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6001 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6061 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6121 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6181 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6241 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6301 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6361 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6421 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6481 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6541 cgggatcact ctcggcatgg acgagctgta caagacaagg agacgacctt ccatgaccga
     6601 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6661 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6721 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6781 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6841 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6901 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6961 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7021 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7081 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7141 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct
     7201 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7261 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7321 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7381 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7441 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7501 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7561 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7621 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7681 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7741 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7801 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7861 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7921 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7981 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     8041 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     8101 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     8161 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     8221 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     8281 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     8341 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     8401 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     8461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8641 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8701 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     8761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10921 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11041 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11101 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11161 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11221 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11281 tagt
//
