LOCUS       V013530                 9411 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013530
VERSION     V013530
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    2793..2873
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             2875..3591
                     /label="AcGFP1"
                     /note="Aequorea coerulescens GFP"
     promoter        4246..4745
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4766..5362
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5379..5967
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6174..6807
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6936..6952)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6960..6976)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6984..7014)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7029..7050)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7338..7926)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8100..8957)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8958..9062)
                     /label="AmpR promoter"
     polyA_signal    9110..9244
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcatggtg
     2881 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat
     2941 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag
     3001 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg
     3061 accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac
     3121 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag
     3181 gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat
     3241 cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg
     3301 gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc
     3361 aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac
     3421 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg
     3481 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc
     3541 ggcttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtgagcggcc
     3601 gctttggaaa gctaggagct gtacaggcgg tttcctgcga aagacagtag ttgtatagcg
     3661 ggggaaggca gaggttgtat aggctgttgg gggaaagcta gaggttgtac agcgctttta
     3721 gaaaggcagc agtcgtatag gacattggaa agctaggggc cgtataggat ttccgggaag
     3781 gcaatgattg tatagtcaaa aaggggaaag acagtgactg tatagaacgg acgcaaggca
     3841 gtgcctgtac aggatttcca gcaaggcagt acttgcgcag tcaaaaaggg ggaaagtagt
     3901 agttgtatag tttggaaagc taggctgctg tacaggcggt ttcctgcgaa agacagagat
     3961 tgtatagcgg gggaaggcag ttagttgtat aggctgttgg gggaaagcta ggaggttgta
     4021 cagcgctttt agaaaggcag ccggtcgtat aggacattgg aaagctaggc tgccgtatag
     4081 gatttccggg aaggcaatcc attgtatagt caaaaagggg aaagacagcc gactgtatag
     4141 aacggacgca aggcaggtgc ctgtacagga tttccagcaa ggcaggggct tgcgcagtca
     4201 aaaaggggga aagtagccaa gttgtatagt ctagataatt ctaccgggta ggggaggcgc
     4261 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     4321 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     4381 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     4441 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     4501 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     4561 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     4621 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     4681 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     4741 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     4801 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4861 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4921 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4981 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5041 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5101 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5161 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5221 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5281 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5341 catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa
     5401 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5461 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5521 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5581 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5641 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5701 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5761 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     5821 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5881 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5941 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     6001 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     6061 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     6121 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     6181 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     6241 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     6301 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     6361 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6421 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     6481 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     6541 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     6601 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6781 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6841 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta
     6901 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7141 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7201 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7261 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7741 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7861 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7921 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7981 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8041 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8161 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9061 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
     9121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9241 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9301 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9361 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
