LOCUS       Exported               11113 bp DNA     circular SYN 15-JUL-2025
DEFINITION  Exported.
ACCESSION   V012792
VERSION     .
KEYWORDS    pLVTHM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11113)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11113)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2023..2047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1911..1952
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     promoter        2147..3325
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    3373..3490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3581..4297
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    4372..4960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     protein_bind    5128..5398
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        5427..5641
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     protein_bind    complement(5676..5709)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5729..5909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5934..5952)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6740..6844
                     /label=AmpR promoter
     CDS             6845..7702
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7876..8464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8710..9039
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          10173..10238
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10368..10388
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    10813..10947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcga tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa
     2101 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2161 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2221 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2281 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2341 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2401 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
     3361 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3481 tcaaaatttt atcgatcacg agactagcct cgaggtttaa actacgggat cttcgaaggc
     3541 ctaagcttac gcgcgcgtcc tagcgctacc ggtcgccacc atggtgagca agggcgagga
     3601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3661 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3781 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4021 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4081 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4201 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4261 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgactagc
     4321 tagtagctag ctagctagtc gagctcaagc ttcggggact agtcatatga taatcaacct
     4381 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4441 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4501 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4561 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4621 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4681 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4741 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4801 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4861 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4921 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcggtacgt atggccaggt
     4981 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5041 ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg
     5101 ctagtggatc cccctcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     5161 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     5221 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     5281 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     5341 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     5401 agtgaaagtc ggggctgcag gaattcgaac gctgacgtca tcaacccgct ccaaggaatc
     5461 gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg
     5521 ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa
     5581 atcaccataa acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca
     5641 cgcgtctcca agcttatcga taccgtcgca tgggaataac ttcgtatagc atacattata
     5701 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5881 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5941 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     6001 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     6061 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     6121 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     6181 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     6241 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     6301 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     6361 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6421 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6481 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6541 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6601 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6661 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7981 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8641 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8701 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8761 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8821 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8881 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8941 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9001 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     9061 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     9121 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     9181 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     9241 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     9301 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     9361 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     9421 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     9481 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     9541 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     9601 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     9661 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     9721 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     9781 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     9841 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     9901 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     9961 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
    10021 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    10081 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
    10141 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
    10201 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
    10261 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
    10321 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
    10381 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    10441 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    10501 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    10561 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    10621 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    10681 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    10741 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    10801 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10921 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10981 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    11041 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    11101 acaaacttag tag
//
