LOCUS       V013666                 9308 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013666
VERSION     V013666
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2818..2883
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             2890..3999
                     /gene="TPST1"
                     /label="Protein-tyrosine sulfotransferase 1"
                     /note="Protein-tyrosine sulfotransferase 1 from Homo
                     sapiens. Accession#: O60507"
     misc_feature    4011..4597
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4617..5213
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5230..5818
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6025..6658
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6786..6802)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6810..6826)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6834..6864)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6879..6900)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7188..7776)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7950..8807)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8808..8912)
                     /label="AmpR promoter"
     polyA_signal    8960..9094
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac
     2821 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     2881 aagctcgaga tggttggaaa gctgaagcag aacttactat tggcatgtct ggtgattagt
     2941 tctgtgactg tgttttacct gggccagcat gccatggaat gccatcaccg gatagaggaa
     3001 cgtagccagc cagtcaaatt ggagagcaca aggaccactg tgagaactgg cctggacctc
     3061 aaagccaaca aaacctttgc ctatcacaaa gatatgcctt taatatttat tggaggtgtg
     3121 cctcggagtg gaaccacact catgagggcc atgctggacg cacatcctga cattcgctgt
     3181 ggagaggaaa ccagggtcat tccccgaatc ctggccctga agcagatgtg gtcacggtca
     3241 agtaaagaga agatccgcct ggatgaggct ggtgttactg atgaagtgct ggattctgcc
     3301 atgcaagcct tcttactaga aattatcgtt aagcatgggg agccagcccc ttatttatgt
     3361 aataaagatc cttttgccct gaaatcttta acttaccttt ctaggttatt ccccaatgcc
     3421 aaatttctcc tgatggtccg agatggccgg gcatcagtac attcaatgat ttctcgaaaa
     3481 gttactatag ctggatttga tctgaacagc tatagggact gtttgacaaa gtggaatcgt
     3541 gctatagaga ccatgtataa ccagtgtatg gaggttggtt ataaaaagtg catgttggtt
     3601 cactatgaac aacttgtctt acatcctgaa cggtggatga gaacactctt aaagttcctc
     3661 cagattccat ggaaccactc agtattgcac catgaagaga tgattgggaa agctggggga
     3721 gtgtctctgt caaaagtgga gagatctaca gaccaagtaa tcaagccagt caatgtagga
     3781 gctctatcaa aatgggttgg gaagataccg ccagatgttt tacaagacat ggcagtgatt
     3841 gctcctatgc ttgccaagct tggatatgac ccatatgcca acccacctaa ctacggaaaa
     3901 cctgatccca aaattattga aaacactcga agggtctata agggagaatt ccaactacct
     3961 gactttctta aagaaaaacc acagactgag caagtggagt agggatcccg cccctctccc
     4021 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4081 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     4141 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     4201 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     4261 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     4321 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     4381 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     4441 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     4501 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     4561 ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga
     4621 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     4681 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     4741 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4801 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4861 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4921 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4981 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5041 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5101 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5161 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc
     5221 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5821 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     5881 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5941 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6001 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     6061 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     6121 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     6181 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     6241 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     6301 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     6361 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     6421 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     6481 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6541 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6601 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6661 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6721 agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa
     6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8941 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9001 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9061 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9121 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     9181 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9241 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac
     9301 ttagtagt
//
