LOCUS       V012180                10234 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012180
VERSION     V012180
KEYWORDS    pLV6-Bmal-luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10234)
  TITLE     Steven Brown plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10234)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             2954..4603
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             5744..5785
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        5827..6156
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6204..6251
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6270..6665
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             6755..6988
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7060..7194
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7221..7356
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7377..7395)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7405..7422)
                     /label="M13 Forward"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(7405..7421)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(7414..7436)
                     /label="M13/pUC Forward"
                     /note="In lacZ gene"
     rep_origin      7563..8018
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8044..8148
                     /label="AmpR promoter"
     CDS             8149..9006
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9180..9768
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     9922..9939
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    10056..10077
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10092..10122
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10130..10146
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10154..10170
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10191..10209
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggat
     1801 ctcgagctca agcttcgaat tcgcccttgg gctacaacag aacaactaac cacccaaaca
     1861 aacaactttg caacttttct ttctttcccg ctcctttgat acttttgcaa agtacatttg
     1921 ctagggccat gtccataaca tgtaatagaa tcttgctcag gtctcaccct gtggccccgg
     1981 ctagctcagt actcgcgatt atgcccctgc ctcagcttct tgagggttgg aattacagac
     2041 tacgccacca cccccgggag acttctcttc cgatcgggaa tgacagctcc actggacgct
     2101 tggaggtcaa gagaaaaaaa aaaaaaaaaa aaaaaaagaa cgcgcgatcc ggttagtcgt
     2161 cggaaggaaa agtcttctaa atgcgctggc tattagcgct gtggttccga ctaggacaag
     2221 ccaggggttt caggaaggtt ctgtcactct gtgttcctaa tatgtggttt cctagatgga
     2281 gctggagagg gatgggcgaa gagatgcggc gttttctcag tgttctgcga gccaggaagg
     2341 ggacggagga ggagggagag ctgagaggca gtcactagcc agggacgcgg ctgaggagca
     2401 ggggacaacg gcgagctcgc agagtccgca acgcagtggc ctcagcgagc tttagacctg
     2461 agggggaaaa ccgagggctg gggcaagaaa tccacagagc gtgccaattg gtccactcct
     2521 cggggcgtgt gcttctgtgc gccaaatgat tggtgaaggg aaagtagcag gtaaaccagc
     2581 cctgccgtct tgccattggt cagaggcttt cctatcggtc actcggttgg ctagcctaac
     2641 gcagagcaga acgcgaattg gtttgggttg tccgccaaga caactccgtt cgctctctct
     2701 gattggctaa cgggaagagg caggtatccg ggctgcgcgg ctcctccatt ggtgggcggg
     2761 gaaggggggt tgggcacagc gattggtggg cggggggccg ggcctgggcc ggcggggagc
     2821 ggattggtcg gaaagtaggt tagtggtgcg acatttaggg aaggcagaaa gtaggtcagg
     2881 gacggaggtg cctgtttacc cgcgccggac ttgccgccgc cgccgccgcg ggatcgatcc
     2941 accggtcgcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     3001 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     3061 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
     3121 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     3181 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     3241 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     3301 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
     3361 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
     3421 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     3481 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
     3541 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
     3601 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     3661 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     3721 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
     3781 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
     3841 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     3901 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     3961 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     4021 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     4081 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     4141 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     4201 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     4261 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     4321 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     4381 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     4441 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     4501 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     4561 agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaagcgg ccgccagtgt
     4621 gatggatatc tgcagaattc gcccttagac actacatttg ctttggcaac agctgcagta
     4681 tcaaagtgca ttaatggtga agttttacag tctgtgaagc ttactggata gagagagaac
     4741 agcttttatg tactgactcc ataaaagcca cctcagagcc attgatacaa gtcaatctac
     4801 catgtgtaac ttcagacaaa gtggaactaa acctgctcca gtgtttcctc atcattgagt
     4861 ttgggctagc tgtggatagc ttgcattaat tgtatatttt ggattttgtt tgtgttgaat
     4921 tttttaatca ttgtgcacag aatcatcatc ggtagctttt atatgcaaaa tggccatttc
     4981 agatgtatgg tgtttttaca ctacaaagaa gtcccccatg tggatatttc ttatactaat
     5041 tgtatcataa agctgtttat tcttccttgt aagaatcctt tactataaat atgggttaaa
     5101 gtataatgta ttagacagtt aaatattttt aataaatgtt tcccttgttc tataagtact
     5161 gttcccaatt gataattagg aagaaaatct tcatatgcta cagggctggt tcatccactt
     5221 caccagcggt ctaaacacat tcctgctgtt atgtttacaa tgtccttctg ctcttataat
     5281 gacttcttcc atgctaccag cttagacata ttataaaaat agctcacttc caaactgtag
     5341 ccacctcaac ttatatgaaa acaaagatat caactccaat ttccagtgct tagatttcaa
     5401 gattacaagc tggcttctct agacctgcag ttacatggtg tgctcaagtc taccaggggt
     5461 agatgtaagc gacggcatct gctctctctt tctatggtcc catcgacaaa ctgagatgtg
     5521 atcccaccaa actaaagttc actctggaaa ccaatgggtt ggtttactgg gcttccatac
     5581 agacacaggc gagaggttcc ctacaggtat gctcctactc aaaggccaca ctagaaagcc
     5641 tttactgggg ctggaagggc gaattccagc acactggcgg ccgttactag tggatccgaa
     5701 ttcgccagca cagtggtcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     5761 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagtttgga attaattctg
     5821 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5881 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6001 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6061 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6121 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6181 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     6241 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     6301 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     6361 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     6421 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     6481 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     6541 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     6601 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     6661 agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6721 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6781 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6961 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7021 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7201 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7261 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7321 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     7381 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7441 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7501 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7561 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7621 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7681 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7741 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7801 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7861 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7921 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7981 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     8041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8161 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8221 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8281 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8341 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8401 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8461 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8521 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8581 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8641 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8701 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8761 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8821 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8881 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8941 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9001 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9841 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9901 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9961 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10021 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    10081 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    10141 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    10201 cactaaaggg aacaaaagct ggagctgcaa gctt
//
