LOCUS       .                       9863 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    227..352
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    845..1078
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1263..1307
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1456..1497
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1574..1690
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             2431..4080
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        4091..4590
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4611..5207
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    complement(5244..5365)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    5479..6067
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6154..6387
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6459..6593
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6620..6755
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6776..6794)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6804..6820)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6962..7417
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7443..7547
                     /label="AmpR promoter"
     CDS             7548..8405
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8579..9167
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9455..9476
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9491..9521
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9529..9545
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     9553..9569
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9590..9608
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9636..9862
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
       61 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      661 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1141 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1561 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
     1681 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1741 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1801 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1861 cacttggcag tacatcaagt gtatcatatg actagcaaaa taggctgtcc ccagtgcaag
     1921 tgcaggtgcc agaacatttc tctatcgata ggtacctctg ccggctgtga gttgcgcagc
     1981 ggccaagcac cattcccccg tgccgcagtg gtacgcgcca ctccggggct gcacgagcgg
     2041 gccaccgccg tgccaggtga atggaagtcc cgcaggccgg aagtggacga gcctactctc
     2101 ccgggcgcgg gggggcgcaa gagcgcgcag catcttcatt gaggaacccg ggcggcgaac
     2161 atggagttcc atgtgcgtct tatgtaaaga gagcgacggg cgtctccacc aattgatgag
     2221 cgtagctctc aggttccgcc ccgccagtat gcaaatgagg tggcactccg accaatggcg
     2281 cgcgcagggg cgggctcagc gcgcgcggtc acgttttcca ctatgtgaca gcggagggcg
     2341 acgcggcggc agcggcgcta ctgggactag cggtctcgag atctgcgatc taagtaagct
     2401 tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
     2461 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     2521 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     2581 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     2641 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     2701 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     2761 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     2821 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     2881 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     2941 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     3001 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     3061 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     3121 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     3181 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     3241 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     3301 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     3361 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     3421 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     3481 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     3541 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     3601 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     3661 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     3721 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     3781 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
     3841 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     3901 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     3961 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     4021 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
     4081 taattctaga gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     4141 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     4201 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     4261 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     4321 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     4381 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     4441 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     4501 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     4561 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     4621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5161 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcggggcgg
     5221 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5281 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5341 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5401 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     5461 cgataaggat ccgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5881 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6001 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6061 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6121 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6181 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6241 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6301 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6361 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6421 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6481 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6541 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6721 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6781 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6841 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6901 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6961 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7141 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7201 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7261 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7321 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7381 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9481 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9541 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     9601 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     9661 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     9721 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     9781 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     9841 gtgcctagct cgatacataa acg
//
