LOCUS       .                       8013 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             516..1223
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1299..1895
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             2026..2259
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2331..2465
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2492..2627
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2648..2666)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2676..2692)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2834..3289
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3315..3419
                     /label="AmpR promoter"
     CDS             3420..4277
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      4451..5039
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5327..5348
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5363..5393
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5401..5417
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     5425..5441
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5462..5480
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5508..5734
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    5962..6087
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6580..6813
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6998..7042
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7191..7232
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     promoter        7322..7769
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kügler et al., 2003)"
     misc_feature    7844..7961
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        8010..8520
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccatggt gagcaagggc gaggaggata
      541 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      601 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      661 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      721 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      781 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      841 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      901 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      961 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1021 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1081 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1141 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1201 gcggcatgga cgagctgtac aaggaatttg gcagcggcca gtgcaccaac tatgccctgc
     1261 tgaaactggc tggcgatgtg gaaagcaacc ccggacccat gaccgagtac aagcccacgg
     1321 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     1381 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     1441 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     1501 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     1561 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     1621 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     1681 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     1741 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     1801 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     1861 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     1921 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca
     1981 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2041 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     2101 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2161 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2221 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     2281 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2461 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2581 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     2641 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     2701 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2761 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2821 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2881 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2941 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3001 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3061 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3121 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3181 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3241 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     3301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3481 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3541 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3601 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3661 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3721 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3781 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3841 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3901 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3961 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4021 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4081 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4141 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4201 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4261 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4321 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4381 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4561 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5101 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5161 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5221 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5281 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5341 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5401 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     5461 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     5521 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     5581 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     5641 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     5701 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     5761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5881 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     5941 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6001 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6061 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6121 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     6181 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6241 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6301 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6361 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     6421 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     6481 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     6541 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     6601 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     6661 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     6721 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     6781 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     6841 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     6901 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     6961 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7021 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7081 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7141 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7201 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     7261 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     7321 aagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg
     7381 accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag
     7441 agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc
     7501 ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg
     7561 cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc
     7621 gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc
     7681 gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg
     7741 gaggagtcgt gtcgtgcctg agagcgcagt cgagaaacac cggtccgcag gtatgcacgc
     7801 gtgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     7861 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7921 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7981 gcagagatcc actttggccg cggctcgagg ggg
//
