LOCUS       V014246                 8366 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014246
VERSION     V014246
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..388
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             397..1188
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             1319..1552
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1624..1758
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(1941..1959)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1969..1985)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2127..2582
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2608..2712
                     /label="AmpR promoter"
     CDS             2713..3570
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3744..4332
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4620..4641
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4656..4686
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4694..4710
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4718..4734
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4755..4773
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4801..5027
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    5255..5380
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5873..6106
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6291..6335
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6484..6525
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        6633..6873
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        6949..7252
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7253..7456
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             7491..8207
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    8249..8366
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg ggttgggggt
       61 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      301 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      361 cttttttgga ggcctaggct tttgcaaaac gcgaccatgg ctgaacaaga tggattgcac
      421 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      481 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      541 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      601 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      661 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      721 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      781 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      841 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      901 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
      961 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     1021 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1081 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1141 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg acgcccgccc
     1201 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga
     1261 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     1321 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     1381 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1441 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1501 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     1561 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     1621 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1681 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1741 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     1801 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1861 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1921 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     1981 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2041 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2101 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     2161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2521 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2581 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4501 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4561 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4621 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     4681 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     4741 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     4801 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     4861 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     4921 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     4981 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     5041 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5101 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5161 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     5221 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     5281 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     5341 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     5401 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     5461 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     5521 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     5581 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     5641 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     5701 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     5761 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     5821 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     5881 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     5941 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6001 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     6061 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     6121 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     6181 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     6241 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     6301 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     6361 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     6421 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     6481 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     6541 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     6601 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     6661 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     6721 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     6781 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     6841 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggcaacaag atgaagagca
     6901 ccaattcaag agattggtgc tcttcatctt gttgtttttt gaattccgcg ttacataact
     6961 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     7021 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7081 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7141 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     7201 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     7261 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     7321 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     7381 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     7441 ctatataagc agagctaatc gtcgacgatt cgtcagaatc ggtcgccacc atggtgagca
     7501 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7561 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7621 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7681 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7741 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7801 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7861 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7921 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7981 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     8041 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     8101 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     8161 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ttctcgacct
     8221 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     8281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     8341 aacaaattac aaaaattcaa aatttt
//
