LOCUS       .                      13326 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        270..345
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        758..969
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     CDS             994..5097
                     /label="Cas9"
                     /note="S. pyogenes Cas9, codon optimized for C. elegans
                     with synthetic introns"
     CDS             5146..5169
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5236..5952
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    6629..7217
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7289..7522
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7554..7778
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7824..8252
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8395..8528
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8613..8643)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8658..8679)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8967..9555)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9729..10586)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(10587..10691)
                     /label="AmpR promoter"
     enhancer        10957..11336
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11337..11539
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             11554..11734
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11781..11906
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12399..12632
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12817..12861
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             13010..13051
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    13159..13276
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gaaccggaga gtaggtcggg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgag
      361 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      421 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      481 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      541 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      601 aacaccgcct cttttcaatc tgactggttt tagagctaga aatagcaagt taaaataagg
      661 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgc tagctaggtc
      721 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      781 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
      841 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      901 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      961 agaacacagg accggttcta gagcgctgcc accatggaca agaagtacag catcggcctg
     1021 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     1081 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     1141 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     1201 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     1261 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     1321 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     1381 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     1441 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     1501 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     1561 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     1621 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     1681 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     1741 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     1801 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     1861 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     1921 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     1981 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     2041 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     2101 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     2161 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     2221 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     2281 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     2341 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     2401 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     2461 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     2521 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     2581 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     2641 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     2701 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     2761 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     2821 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     2881 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     2941 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     3001 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     3061 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     3121 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     3181 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     3241 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     3301 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     3361 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     3421 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     3481 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     3541 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     3601 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     3661 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     3721 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     3781 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     3841 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     3901 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     3961 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     4021 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     4081 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     4141 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     4201 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     4261 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     4321 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     4381 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     4441 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     4501 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     4561 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     4621 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     4681 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     4741 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     4801 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     4861 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     4921 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     4981 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     5041 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaag
     5101 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagatta caaagacgat
     5161 gacgataagg gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa
     5221 gagaatcctg gaccgatggt gtctaagggc gaagagctgt tcaccggggt ggtgcccatc
     5281 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     5341 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     5401 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     5461 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5521 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5581 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5641 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5701 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5761 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5821 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5881 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5941 gagctgtaca aggagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6001 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6061 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6121 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6181 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6241 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6301 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6361 aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt
     6421 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6481 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6541 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6601 gcctgactcg agtacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6661 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6721 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6781 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6841 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6901 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6961 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7021 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7081 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7141 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7201 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     7261 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7321 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7381 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7441 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7501 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7561 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7621 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7681 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7741 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7801 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7861 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7921 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7981 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8041 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8101 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8161 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8221 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatcacgt gctacgagat
     8281 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8341 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8521 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8581 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8641 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8701 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8761 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8941 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9121 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9181 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9241 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9301 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9361 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9421 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9481 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9601 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9661 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9721 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9781 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9841 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9901 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9961 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10021 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10081 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10141 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10201 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10261 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10321 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10381 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10441 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10501 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10561 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10621 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10681 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    10741 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10801 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    10861 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    10921 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    10981 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    11041 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    11101 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    11161 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
    11221 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
    11281 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
    11341 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
    11401 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
    11461 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
    11521 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
    11581 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11641 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11701 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    11761 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    11821 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    11881 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    11941 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    12001 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    12061 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    12121 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    12181 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    12241 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    12301 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12361 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12421 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12481 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12541 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    12601 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    12661 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    12721 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    12781 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    12841 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    12901 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12961 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    13021 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13081 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
    13141 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    13201 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    13261 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
    13321 ggtacc
//
