LOCUS       V015024                 8053 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015024
VERSION     V015024
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             516..1223
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1299..2090
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             2221..2454
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2526..2660
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2687..2822
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2843..2861)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2871..2887)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3029..3484
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3510..3614
                     /label="AmpR promoter"
     CDS             3615..4472
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4646..5234
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5522..5543
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5558..5588
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5596..5612
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5620..5636
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5657..5675
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5703..5929
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5930..6110
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6157..6282
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6775..7008
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7193..7237
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7386..7427
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        7535..7775
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    7884..8001
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        8050..8053
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccatggt gagcaagggc gaggaggata
      541 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      601 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      661 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      721 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      781 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      841 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      901 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      961 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1021 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1081 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1141 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1201 gcggcatgga cgagctgtac aaggaatttg gcagcggcca gtgcaccaac tatgccctgc
     1261 tgaaactggc tggcgatgtg gaaagcaacc ccggacccat ggctgaacaa gatggattgc
     1321 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1381 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1441 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1501 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1561 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1621 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1681 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1741 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1801 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1861 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1921 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1981 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     2041 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgacgcccgc
     2101 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
     2161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     2221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     2281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     2341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     2401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     2461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     2521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     2701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2821 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     2881 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2941 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3001 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3481 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     5641 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     5701 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     5761 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     5821 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     5881 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     5941 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6001 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6061 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6121 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     6181 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     6241 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     6301 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     6361 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     6421 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     6481 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     6541 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     6601 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     6661 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     6721 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     6781 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     6841 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     6901 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     6961 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7021 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7081 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7141 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7201 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7261 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7321 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7381 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     7441 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc
     7501 acgagactag cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc
     7561 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     7621 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     7681 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     7741 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggcaaca agatgaagag
     7801 caccaattca agagattggt gctcttcatc ttgttgtttt ttgaattctc gacctcgaga
     7861 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     7921 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7981 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     8041 cggctcgagg ggg
//
