LOCUS       V014978                11171 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014978
VERSION     V014978
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2807..3514
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             3536..5917
                     /gene="FURIN"
                     /label="Furin"
                     /note="Furin from Homo sapiens. Accession#: P09958"
     CDS             5918..5935
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        6006..6505
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6526..7122
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7139..7727
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7934..8567
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8744..8774)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8789..8810)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9098..9686)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9860..10717)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10718..10822)
                     /label="AmpR promoter"
     polyA_signal    10870..11004
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg
     2821 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2881 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2941 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3001 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3061 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3121 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3181 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3241 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3301 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3361 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3421 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3481 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gagtcatgga
     3541 gctgaggccc tggttgctat gggtggtagc agcaacagga accttggtcc tgctagcagc
     3601 tgatgctcag ggccagaagg tcttcaccaa cacgtgggct gtgcgcatcc ctggaggccc
     3661 agcggtggcc aacagtgtgg cacggaagca tgggttcctc aacctgggcc agatcttcgg
     3721 ggactattac cacttctggc atcgaggagt gacgaagcgg tccctgtcgc ctcaccgccc
     3781 gcggcacagc cggctgcaga gggagcctca agtacagtgg ctggaacagc aggtggcaaa
     3841 gcgacggact aaacgggacg tgtaccagga gcccacagac cccaagtttc ctcagcagtg
     3901 gtacctgtct ggtgtcactc agcgggacct gaatgtgaag gcggcctggg cgcagggcta
     3961 cacagggcac ggcattgtgg tctccattct ggacgatggc atcgagaaga accacccgga
     4021 cttggcaggc aattatgatc ctggggccag ttttgatgtc aatgaccagg accctgaccc
     4081 ccagcctcgg tacacacaga tgaatgacaa caggcacggc acacggtgtg cgggggaagt
     4141 ggctgcggtg gccaacaacg gtgtctgtgg tgtaggtgtg gcctacaacg cccgcattgg
     4201 aggggtgcgc atgctggatg gcgaggtgac agatgcagtg gaggcacgct cgctgggcct
     4261 gaaccccaac cacatccaca tctacagtgc cagctggggc cccgaggatg acggcaagac
     4321 agtggatggg ccagcccgcc tcgccgagga ggccttcttc cgtggggtta gccagggccg
     4381 aggggggctg ggctccatct ttgtctgggc ctcggggaac gggggccggg aacatgacag
     4441 ctgcaactgc gacggctaca ccaacagtat ctacacgctg tccatcagca gcgccacgca
     4501 gtttggcaac gtgccgtggt acagcgaggc ctgctcgtcc acactggcca cgacctacag
     4561 cagtggcaac cagaatgaga agcagatcgt gacgactgac ttgcggcaga agtgcacgga
     4621 gtctcacacg ggcacctcag cctctgcccc cttagcagcc ggcatcattg ctctcaccct
     4681 ggaggccaat aagaacctca catggcggga catgcaacac ctggtggtac agacctcgaa
     4741 gccagcccac ctcaatgcca acgactgggc caccaatggt gtgggccgga aagtgagcca
     4801 ctcatatggc tacgggcttt tggacgcagg cgccatggtg gccctggccc agaattggac
     4861 cacagtggcc ccccagcgga agtgcatcat cgacatcctc accgagccca aagacatcgg
     4921 gaaacggctc gaggtgcgga agaccgtgac cgcgtgcctg ggcgagccca accacatcac
     4981 tcggctggag cacgctcagg cgcggctcac cctgtcctat aatcgccgtg gcgacctggc
     5041 catccacctg gtcagcccca tgggcacccg ctccaccctg ctggcagcca ggccacatga
     5101 ctactccgca gatgggttta atgactgggc cttcatgaca actcattcct gggatgagga
     5161 tccctctggc gagtgggtcc tagagattga aaacaccagc gaagccaaca actatgggac
     5221 gctgaccaag ttcaccctcg tactctatgg caccgcccct gaggggctgc ccgtacctcc
     5281 agaaagcagt ggctgcaaga ccctcacgtc cagtcaggcc tgtgtggtgt gcgaggaagg
     5341 cttctccctg caccagaaga gctgtgtcca gcactgccct ccagggttcg ccccccaagt
     5401 cctcgatacg cactatagca ccgagaatga cgtggagacc atccgggcca gcgtctgcgc
     5461 cccctgccac gcctcatgtg ccacatgcca ggggccggcc ctgacagact gcctcagctg
     5521 ccccagccac gcctccttgg accctgtgga gcagacttgc tcccggcaaa gccagagcag
     5581 ccgagagtcc ccgccacagc agcagccacc tcggctgccc ccggaggtgg aggcggggca
     5641 acggctgcgg gcagggctgc tgccctcaca cctgcctgag gtggtggccg gcctcagctg
     5701 cgccttcatc gtgctggtct tcgtcactgt cttcctggtc ctgcagctgc gctctggctt
     5761 tagttttcgg ggggtgaagg tgtacaccat ggaccgtggc ctcatctcct acaaggggct
     5821 gccccctgaa gcctggcagg aggagtgccc gtctgactca gaagaggacg agggccgggg
     5881 cgagaggacc gcctttatca aagaccagag cgccctccat caccatcatc accattgaat
     5941 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatt
     6001 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     6061 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     6121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     6181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     6241 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     6301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     6361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6481 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6721 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6901 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6961 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa
     7141 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7201 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7261 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7321 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7381 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7441 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7501 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7561 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7621 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7681 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     7741 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7801 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7861 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7921 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7981 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     8041 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     8101 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     8161 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     8221 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     8281 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     8341 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     8401 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8461 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8521 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8581 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8641 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8701 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8761 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8821 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8881 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8941 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9481 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9721 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10141 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10441 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10741 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10801 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10861 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10981 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    11041 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    11101 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    11161 aacttagtag t
//
