LOCUS       V014862                12976 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014862
VERSION     V014862
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        271..346
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        408..619
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             644..4747
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4796..4819
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4886..5602
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    6279..6867
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6939..7172
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7204..7428
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7474..7902
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8045..8178
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8263..8293)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8308..8329)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8617..9205)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9379..10236)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10237..10341)
                     /label="AmpR promoter"
     enhancer        10607..10986
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10987..11189
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             11204..11384
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11431..11556
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12049..12282
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12467..12511
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             12660..12701
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    12809..12926
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg caacaagatg aagagcacca gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
      361 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
      421 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
      481 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
      541 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
      601 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaca agaagtacag
      661 catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      721 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      781 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      841 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      901 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      961 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     1021 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     1081 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     1141 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     1201 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     1261 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     1321 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     1381 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     1441 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     1501 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     1561 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     1621 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     1681 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     1741 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     1801 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     1861 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     1921 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     1981 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     2041 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     2101 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     2161 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     2221 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     2281 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     2341 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     2401 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     2461 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     2521 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     2581 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     2641 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     2701 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     2761 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     2821 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     2881 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     2941 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     3001 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     3061 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     3121 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     3181 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     3241 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     3301 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     3361 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     3421 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     3481 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     3541 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     3601 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     3661 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     3721 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     3781 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     3841 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     3901 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     3961 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     4021 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     4081 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     4141 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     4201 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     4261 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     4321 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     4381 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     4441 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     4501 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     4561 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     4621 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     4681 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     4741 aggcgacaag cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagatta
     4801 caaagacgat gacgataagg gatccggcgc aacaaacttc tctctgctga aacaagccgg
     4861 agatgtcgaa gagaatcctg gaccgatggt gtctaagggc gaagagctgt tcaccggggt
     4921 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4981 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5041 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5101 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5161 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5221 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5281 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5341 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5401 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5461 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5521 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5581 cggcatggac gagctgtaca aggagggcag aggaagtctt ctaacatgcg gtgacgtgga
     5641 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5701 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5761 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5821 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5881 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5941 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6001 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
     6061 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6121 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6181 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6241 caagcccggt gcctgactcg agtacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     6301 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6361 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6421 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6481 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6541 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6601 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6661 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6721 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6781 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6841 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     6901 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6961 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7141 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7201 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7261 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7321 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7381 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7441 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7501 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7561 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7621 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7681 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7741 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7801 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7861 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatcacgt
     7921 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7981 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8041 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8101 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8161 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8221 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8281 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8341 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8401 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8461 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8521 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8581 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9241 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9301 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9361 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9421 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9481 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9541 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9601 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9661 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9721 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9781 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9841 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9901 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9961 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10021 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10081 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10141 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10201 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10261 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10321 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10381 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10441 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10501 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    10561 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
    10621 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    10681 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    10741 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    10801 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    10861 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    10921 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    10981 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
    11041 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
    11101 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
    11161 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
    11221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    11281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    11341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    11401 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    11461 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    11521 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    11581 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    11641 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    11701 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    11761 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    11821 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    11881 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    11941 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    12001 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    12061 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    12121 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    12181 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    12241 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    12301 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    12361 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    12421 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    12481 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    12541 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    12601 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    12661 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    12721 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
    12781 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    12841 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    12901 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
    12961 ggttaattaa ggtacc
//
