LOCUS       V015052                12194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015052
VERSION     V015052
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        266..341
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        403..614
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             639..4742
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4791..4814
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4821..4874
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4875..5471
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5497..6085
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6157..6390
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6422..6646
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6692..7120
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    7263..7396
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(7481..7511)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7526..7547)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7835..8423)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8597..9454)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9455..9559)
                     /label="AmpR promoter"
     promoter        10205..10407
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             10422..10602
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10649..10774
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    11267..11500
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             11685..11729
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             11878..11919
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    12027..12144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggacg tctctgtttt agagctagaa atagcaagtt aaaataaggc
      301 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttcgctagct
      361 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
      421 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
      481 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      541 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      601 ccgccagaac acaggaccgg ttctagagcg ctgccaccat ggacaagaag tacagcatcg
      661 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
      721 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
      781 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
      841 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
      901 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
      961 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     1021 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     1081 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     1141 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     1201 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     1261 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     1321 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     1381 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     1441 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1501 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1561 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1621 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1681 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     1741 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     1801 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     1861 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     1921 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     1981 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     2041 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     2101 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     2161 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     2221 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     2281 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     2341 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     2401 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     2461 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2521 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2581 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2641 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     2701 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     2761 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     2821 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     2881 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     2941 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     3001 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     3061 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     3121 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     3181 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     3241 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     3301 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     3361 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     3421 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3481 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3541 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3601 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3661 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     3721 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     3781 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     3841 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     3901 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     3961 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     4021 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     4081 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     4141 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     4201 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     4261 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     4321 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     4381 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     4441 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4501 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4561 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4621 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4681 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     4741 acaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa gattacaaag
     4801 acgatgacga taagggatcc gagggcagag gaagtcttct aacatgcggt gacgtggagg
     4861 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4921 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4981 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5041 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5101 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5161 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5221 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg
     5281 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5341 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5401 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5461 agcccggtgc ctgactcgag tacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     5521 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5581 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5641 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5701 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5761 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5821 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5881 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5941 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6001 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6061 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6121 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6181 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6241 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6301 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6361 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     6421 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6481 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6541 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6601 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6661 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6901 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7021 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatcacgtgc
     7141 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7201 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7261 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7381 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7441 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7501 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7621 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7681 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7741 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8461 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8521 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8641 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8701 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8761 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8821 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8881 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8941 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9001 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9061 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9121 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9181 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9241 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9301 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9361 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9421 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9481 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9541 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9601 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9661 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9721 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9781 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     9841 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     9901 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     9961 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    10021 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
    10081 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
    10141 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
    10201 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
    10261 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
    10321 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
    10381 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
    10441 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    10501 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    10561 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
    10621 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
    10681 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    10741 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    10801 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    10861 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    10921 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    10981 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    11041 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    11101 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    11161 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    11221 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    11281 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    11341 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    11401 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    11461 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    11521 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    11581 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    11641 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    11701 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    11761 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    11821 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    11881 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    11941 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
    12001 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    12061 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    12121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    12181 ttaattaagg tacc
//
