LOCUS       V014508                 7983 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014508
VERSION     V014508
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             338..571
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    643..777
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      804..939
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(960..978)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(988..1004)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1146..1601
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1627..1731
                     /label="AmpR promoter"
     CDS             1732..2589
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2763..3351
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3639..3660
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3675..3705
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3713..3729
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3737..3753
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3774..3792
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3820..4046
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4047..4227
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4274..4399
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4892..5125
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5310..5354
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5503..5544
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        5652..5892
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    7814..7931
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtgg ggatccggta cctttaagac caatgactta caaggcagct
      301 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
      361 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
      421 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      481 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      541 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
      601 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
      661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
      781 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      901 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
      961 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1021 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1081 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1141 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1201 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1261 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1321 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1381 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1441 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1501 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1561 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     1621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1681 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1741 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1801 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1861 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1921 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1981 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2041 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2101 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2161 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2221 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2281 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2341 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2401 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2461 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2521 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2581 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2881 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3421 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3481 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3541 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3601 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3661 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3721 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     3781 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     3841 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     3901 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     3961 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     4021 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     4081 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4141 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4201 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     4261 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     4321 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     4381 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     4441 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     4501 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     4561 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     4621 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     4681 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     4741 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     4801 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     4861 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     4921 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     4981 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     5041 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     5101 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     5161 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     5221 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     5281 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     5341 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     5401 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     5461 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     5521 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     5581 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
     5641 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     5701 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     5761 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     5821 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     5881 tgtggaaagg acgaaacacc ggtgtgttgt aaatgagcac acaaaataca catgctaaaa
     5941 tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat
     6001 caataattag aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa
     6061 aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc
     6121 acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct
     6181 aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac
     6241 atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa attctaatcc
     6301 agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata aacaattttc
     6361 cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca tgtcttctaa
     6421 aactaattca taatttttgt cttttaatgc accaatattt aataccatat caatttctgt
     6481 tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg tgcatgcacc
     6541 tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata attctttaca
     6601 atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc
     6661 acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt gatctatata
     6721 tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc tttaataatt
     6781 tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa ataaattttt
     6841 agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc taaataaaat
     6901 tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc aacattaaca
     6961 actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat taatggttca
     7021 atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca
     7081 cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg ttttttataa
     7141 aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc tctagctgag
     7201 tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa tccgttatct
     7261 tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt tacaatatta
     7321 actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa acttgaatat
     7381 agagccggat tttttggaat gctatttaag cgttttagat ttgataattt tcaatcaatt
     7441 aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg ttttattgcc
     7501 attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc atgtttaata
     7561 ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt tacaacattt
     7621 ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata tcctgattca
     7681 cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa aataattttt
     7741 aaatcttttt tagcttcttg ctcttttttg tagaattctc gacctcgaga caaatggcag
     7801 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     7861 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     7921 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
     7981 ggg
//
