LOCUS       V014975                 9733 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014975
VERSION     V014975
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2005..2208
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2288..2359
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags"
     CDS             2366..3841
                     /gene="DYNC1LI2"
                     /label="Cytoplasmic dynein 1 light intermediate chain 2"
                     /note="Cytoplasmic dynein 1 light intermediate chain 2 from
                     Homo sapiens. Accession#: O43237"
     promoter        3892..4103
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             4116..4384
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4440..5105
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             5175..5228
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             5229..5825
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5829..6416
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6490..6723
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6795..6929
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6935..7070
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(7108..7124)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7132..7148)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7156..7186)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7201..7222)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7510..8098)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8272..9129)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9130..9234)
                     /label="AmpR promoter"
     primer_bind     9708..9724
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcatggc gccggtgggg gtggagaaga agctgctgct
     2401 aggtcccaac gggcccgcgg tggcggccgc cggcgacctg accagtgagg aggaggaagg
     2461 ccagagccta tggtcctcca ttctgagcga agtgtccacc cgcgccaggt ccaagctgcc
     2521 gtccggcaag aacatcctgg tcttcggtga agatggttct ggtaaaacaa ccctcatgac
     2581 taaactacaa ggagctgagc atggcaaaaa aggaagaggc ctagaatatc tctacctcag
     2641 tgtccatgat gaggaccgag atgatcacac gcgctgcaac gtgtggattc tggatggaga
     2701 cttgtaccac aaaggcctgc tgaaatttgc agtttctgct gaatccttgc cagagaccct
     2761 cgtcattttt gttgcagaca tgtctagacc ttggactgtg atggaatctc tgcagaaatg
     2821 ggctagtgtt ttacgtgagc acattgataa aatgaaaatt ccaccagaaa aaatgaggga
     2881 gctggaacgg aagtttgtga aagattttca agactatatg gaacctgaag aaggttgtca
     2941 aggttcccca cagagaagag gccctctgac ctcaggctcc gatgaagaaa atgttgccct
     3001 gcctctgggt gacaatgtgc tgactcataa cctggggatc ccggtgttgg tggtgtgcac
     3061 aaagtgtgat gcggtgagtg tcctggagaa ggagcacgat tacagggatg agcatttgga
     3121 ctttatccag tcacacctgc ggaggttctg ccttcagtat ggagctgcct tgatttacac
     3181 atcagtgaaa gaagagaaaa acctcgactt gttgtataag tatattgttc ataaaacata
     3241 cggtttccac ttcaccacac ctgccttagt tgtggaaaag gatgccgttt ttatacctgc
     3301 aggctgggac aatgaaaaga aaatagctat tttacatgaa aattttacaa ccgtgaagcc
     3361 ggaagatgca tatgaagact ttattgtgaa acctcccgtg agaaagctgg tccacgacaa
     3421 agagttggca gcagaagatg agcaggtgtt cctaatgaag caacagtcac tccttgccaa
     3481 gcaaccagcc actcccacga gagcttctga atctcctgca agaggaccct ctggctctcc
     3541 aaggacccag ggtcggggag ggccagccag tgtgcctagc tcctccccag gcacgtcagt
     3601 aaaaaagccg gacccaaaca tcaaaaataa tgcagcaagt gaaggggtgt tggccagctt
     3661 cttcaacagt ctgttgagta aaaagacagg ctctcctgga agtcctggtg ctggtggggt
     3721 gcagagcaca gccaagaagt caggacaaaa gactgtgttg tcaaatgttc aggaagaact
     3781 ggatagaatg actcgaaagc cagactctat ggtaacaaac tcttcaacag aaaatgaagc
     3841 ctgaggatcc gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc
     3901 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct
     3961 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     4021 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     4081 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg
     4141 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg
     4201 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac
     4261 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt
     4321 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca
     4381 gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc
     4441 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc
     4501 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca
     4561 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct
     4621 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg
     4681 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct
     4741 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct
     4801 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc
     4861 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc
     4921 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc
     4981 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc
     5041 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct
     5101 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct
     5161 ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     5221 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5281 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5341 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5401 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5461 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5521 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5581 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5641 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5701 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5761 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5821 gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6241 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6361 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg
     6421 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6481 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     6541 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6721 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6781 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6901 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     6961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7021 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     7081 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7921 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9241 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9301 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9361 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9421 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9481 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9541 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9601 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9661 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9721 cagtgccaag ctg
//
