LOCUS       V014878                 6805 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014878
VERSION     V014878
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             535..930
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             1011..1244
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1316..1450
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1477..1612
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1633..1651)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1661..1677)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1819..2274
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2300..2404
                     /label="AmpR promoter"
     CDS             2405..3262
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3436..4024
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4312..4333
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4348..4378
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4386..4402
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4410..4426
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4447..4465
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4493..4719
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4720..4900
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4947..5072
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5565..5798
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5983..6027
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6176..6217
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        6325..6565
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    6636..6753
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        6802..6805
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccgc caccatggcc
      541 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
      601 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
      661 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
      721 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
      781 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
      841 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
      901 ttgctgccct ctggttatgt gtgggagggc taagtcgacg gtacctttaa gaccaatgac
      961 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     1021 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     1081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     1141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     1201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     1261 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     1321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1441 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     1621 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     1681 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1741 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1801 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1861 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1921 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1981 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2041 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2101 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2161 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     2281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2641 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2941 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4201 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4261 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4321 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4381 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     4441 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     4501 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     4561 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     4621 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     4681 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     4741 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4801 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4861 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     4921 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     4981 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5041 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     5101 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     5161 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     5221 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     5281 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     5341 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     5401 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     5461 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     5521 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     5581 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     5641 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     5701 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     5761 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     5821 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     5881 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     5941 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6001 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6061 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6121 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6181 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     6241 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag
     6301 cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat
     6361 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     6421 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     6481 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     6541 gctttatata tcttgtggaa aggacgaaac accggtccgc aggtatgcac gcgtgaattc
     6601 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     6661 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     6721 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     6781 ccactttggc cgcggctcga ggggg
//
