LOCUS       V014384                12451 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014384
VERSION     V014384
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        266..341
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        403..614
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             646..666
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             676..2391
                     /label="Cas9(N)"
                     /note="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
     CDS             2965..2985
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             5134..5154
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             6361..7077
                     /label="EGFP"
                     /note="enhanced GFP"
     LTR             7152..7385
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7417..7641
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(7738..7768)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7783..7804)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8092..8680)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8854..9711)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9712..9816)
                     /label="AmpR promoter"
     enhancer        10082..10461
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10462..10664
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             10679..10859
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10906..11031
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    11524..11757
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             11942..11986
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             12135..12176
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    12284..12401
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggacg tctctgtttt agagctagaa atagcaagtt aaaataaggc
      301 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttcgctagct
      361 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
      421 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
      481 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      541 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      601 ccgccagaac acaggaccgg ttctagacgt acggccacca tgagccccaa gaagaagaga
      661 aaggtggagg ccagcgacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg
      721 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
      781 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
      841 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
      901 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
      961 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     1021 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     1081 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     1141 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     1201 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     1261 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     1321 agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     1381 ctgttcggca acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     1441 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     1501 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     1561 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     1621 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     1681 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     1741 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     1801 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag
     1861 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     1921 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     1981 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     2041 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     2101 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     2161 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     2221 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     2281 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     2341 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     2401 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     2461 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     2521 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     2581 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     2641 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     2701 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt ctccggactc
     2761 agatctggca gcggtctcga gatggaagtt agcgctctgg aaaaagaagt gtctgcactc
     2821 gagaaagaag taagtgccct tgagaaggag gtgtccgcac tcgagaagga ggtcagcgcc
     2881 ctggaaaagg gaggcggagg ctccatgcag ctgatccacg acgacagcct gacctttaaa
     2941 gaggacatcc agaaagccca ggtggaaaac ctgtactttc agtccccatc atcaaaggtg
     3001 tccgccctga aggaaaaagt aagcgcactg aaagaaaagg tgagcgcgct gaaggagaaa
     3061 gtgagcgccc tgaaagagaa agtctctgcc cttaaggagg gaggcggagg ctcctccggc
     3121 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     3181 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     3241 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     3301 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     3361 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     3421 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     3481 tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     3541 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     3601 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     3661 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     3721 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     3781 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     3841 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     3901 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     3961 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     4021 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     4081 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     4141 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     4201 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     4261 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     4321 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     4381 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     4441 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     4501 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     4561 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     4621 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     4681 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     4741 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     4801 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     4861 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     4921 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     4981 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     5041 tacgagacac ggatcgacct gtctcagctg ggaggcgaca gcgctggagg aggtggaagc
     5101 ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc
     5161 gctggatccg gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac
     5221 gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc
     5281 gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taacgctagc
     5341 ggcagtggac ctacacaggc cggcgagggc acactgtctg aagctctgct gcagctgcag
     5401 ttcgacgacg aggatctggg agccctgctg ggaaacagca ccgatcctgc cgtgttcacc
     5461 gacctggcca gcgtggacaa cagcgagttc cagcagctgc tgaaccaggg catccctgtg
     5521 gcccctcaca ccaccgagcc catgctgatg gaataccccg aggccatcac ccggctcgtg
     5581 acaggcgctc agaggcctcc tgatccagct cctgcccctc tgggagcacc aggcctgcct
     5641 aatggactgc tgtctggcga cgaggacttc agctctatcg ccgatatgga tttctcagcc
     5701 ttgctgggct ctggcagcgg cagccgggat tccagggaag ggatgttttt gccgaagcct
     5761 gaggccggct ccgctattag tgacgtgttt gagggccgcg aggtgtgcca gccaaaacga
     5821 atccggccat ttcatcctcc aggaagtcca tgggccaacc gcccactccc cgccagcctc
     5881 gcaccaacac caaccggtcc agtacatgag ccagtcgggt cactgacccc ggcaccagtc
     5941 cctcagccac tggatccagc gcccgcagtg actcccgagg ccagtcacct gttggaggat
     6001 cccgatgaag aaacgagcca ggctgtcaaa gcccttcggg agatggccga tactgtgatt
     6061 ccccagaagg aagaggctgc aatctgtggc caaatggacc tttcccatcc gcccccaagg
     6121 ggccatctgg atgagctgac aaccacactt gagtccatga ccgaggatct gaacctggac
     6181 tcacccctga ccccggaatt gaacgagatt ctggatacct tcctgaacga cgagtgcctc
     6241 ttgcatgcca tgcatatcag cacaggactg tccatcttcg acacatctct ctttggatcc
     6301 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     6361 atggtgtcta agggcgaaga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6541 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6661 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6721 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6781 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6841 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6901 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6961 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7021 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7081 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7141 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7201 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7261 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7321 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7381 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7441 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7501 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7561 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7621 gggatgcggt gggctctatg gcttctgagg tataccgtcg acctctagct agagcttggc
     7681 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8521 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9841 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     9901 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     9961 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    10021 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
    10081 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
    10141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    10261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
    10501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    10561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
    10621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
    10681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10861 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10921 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10981 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11041 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11101 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11161 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11221 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11281 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11341 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    11401 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    11461 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    11521 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11581 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11641 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11701 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    11761 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    11821 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    11881 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    11941 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    12001 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    12061 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    12121 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    12181 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
    12241 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
    12301 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    12361 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    12421 gcagagatcc agtttggtta attaaggtac c
//
