LOCUS       V014228                13637 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014228
VERSION     V014228
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2608..2848
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        4738..4813
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        4875..5086
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             5111..8008
                     /label="RfxCas13d"
                     /note="RNA-targeting Type VI CRISPR protein from
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
     CDS             8015..8041
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             8060..8116
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             8117..8710
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    8729..9317
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             9389..9622
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9654..9878
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        10366..10695
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        10743..10790
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10809..11180
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    11313..11446
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(11531..11561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11576..11597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11885..12473)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12647..13504)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13505..13609)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
     5101 cgctgccacc atgatcgaaa aaaaaaagtc cttcgccaag ggcatgggcg tgaagtccac
     5161 actcgtgtcc ggctccaaag tgtacatgac aaccttcgcc gaaggcagcg acgccaggct
     5221 ggaaaagatc gtggagggcg acagcatcag gagcgtgaat gagggcgagg ccttcagcgc
     5281 tgaaatggcc gataaaaacg ccggctataa gatcggcaac gccaaattca gccatcctaa
     5341 gggctacgcc gtggtggcta acaaccctct gtatacagga cccgtccagc aggatatgct
     5401 cggcctgaag gaaactctgg aaaagaggta cttcggcgag agcgctgatg gcaatgacaa
     5461 tatttgtatc caggtgatcc ataacatcct ggacattgaa aaaatcctcg ccgaatacat
     5521 taccaacgcc gcctacgccg tcaacaatat ctccggcctg gataaggaca ttattggatt
     5581 cggcaagttc tccacagtgt atacctacga cgaattcaaa gaccccgagc accatagggc
     5641 cgctttcaac aataacgata agctcatcaa cgccatcaag gcccagtatg acgagttcga
     5701 caacttcctc gataacccca gactcggcta tttcggccag gcctttttca gcaaggaggg
     5761 cagaaattac atcatcaatt acggcaacga atgctatgac attctggccc tcctgagcgg
     5821 actgaggcac tgggtggtcc ataacaacga agaagagtcc aggatctcca ggacctggct
     5881 ctacaacctc gataagaacc tcgacaacga atacatctcc accctcaact acctctacga
     5941 caggatcacc aatgagctga ccaactcctt ctccaagaac tccgccgcca acgtgaacta
     6001 tattgccgaa actctgggaa tcaaccctgc cgaattcgcc gaacaatatt tcagattcag
     6061 cattatgaaa gagcagaaaa acctcggatt caatatcacc aagctcaggg aagtgatgct
     6121 ggacaggaag gatatgtccg agatcaggaa aaatcataag gtgttcgact ccatcaggac
     6181 caaggtctac accatgatgg actttgtgat ttataggtat tacatcgaag aggatgccaa
     6241 ggtggctgcc gccaataagt ccctccccga taatgagaag tccctgagcg agaaggatat
     6301 ctttgtgatt aacctgaggg gctccttcaa cgacgaccag aaggatgccc tctactacga
     6361 tgaagctaat agaatttgga gaaagctcga aaatatcatg cacaacatca aggaatttag
     6421 gggaaacaag acaagagagt ataagaagaa ggacgcccct agactgccca gaatcctgcc
     6481 cgctggccgt gatgtttccg ccttcagcaa actcatgtat gccctgacca tgttcctgga
     6541 tggcaaggag atcaacgacc tcctgaccac cctgattaat aaattcgata acatccagag
     6601 cttcctgaag gtgatgcctc tcatcggagt caacgctaag ttcgtggagg aatacgcctt
     6661 tttcaaagac tccgccaaga tcgccgatga gctgaggctg atcaagtcct tcgctagaat
     6721 gggagaacct attgccgatg ccaggagggc catgtatatc gacgccatcc gtattttagg
     6781 aaccaacctg tcctatgatg agctcaaggc cctcgccgac accttttccc tggacgagaa
     6841 cggaaacaag ctcaagaaag gcaagcacgg catgagaaat ttcattatta ataacgtgat
     6901 cagcaataaa aggttccact acctgatcag atacggtgat cctgcccacc tccatgagat
     6961 cgccaaaaac gaggccgtgg tgaagttcgt gctcggcagg atcgctgaca tccagaaaaa
     7021 acagggccag aacggcaaga accagatcga caggtactac gaaacttgta tcggaaagga
     7081 taagggcaag agcgtgagcg aaaaggtgga cgctctcaca aagatcatca ccggaatgaa
     7141 ctacgaccaa ttcgacaaga aaaggagcgt cattgaggac accggcaggg aaaacgccga
     7201 gagggagaag tttaaaaaga tcatcagcct gtacctcacc gtgatctacc acatcctcaa
     7261 gaatattgtc aatatcaacg ccaggtacgt catcggattc cattgcgtcg agcgtgatgc
     7321 tcaactgtac aaggagaaag gctacgacat caatctcaag aaactggaag agaagggatt
     7381 cagctccgtc accaagctct gcgctggcat tgatgaaact gcccccgata agagaaagga
     7441 cgtggaaaag gagatggctg aaagagccaa ggagagcatt gacagcctcg agagcgccaa
     7501 ccccaagctg tatgccaatt acatcaaata cagcgacgag aagaaagccg aggagttcac
     7561 caggcagatt aacagggaga aggccaaaac cgccctgaac gcctacctga ggaacaccaa
     7621 gtggaatgtg atcatcaggg aggacctcct gagaattgac aacaagacat gtaccctgtt
     7681 cagaaacaag gccgtccacc tggaagtggc caggtatgtc cacgcctata tcaacgacat
     7741 tgccgaggtc aattcctact tccaactgta ccattacatc atgcagagaa ttatcatgaa
     7801 tgagaggtac gagaaaagca gcggaaaggt gtccgagtac ttcgacgctg tgaatgacga
     7861 gaagaagtac aacgataggc tcctgaaact gctgtgtgtg cctttcggct actgtatccc
     7921 caggtttaag aacctgagca tcgaggccct gttcgatagg aacgaggccg ccaagttcga
     7981 caaggagaaa aagaaggtgt ccggcaatct tcaactgcct ccacttgaaa gactgacact
     8041 gggtagttcc ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     8101 agagaatcct ggaccgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8161 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8221 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8281 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8341 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8401 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8461 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     8521 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8581 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8641 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8701 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     8761 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8821 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8881 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8941 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9001 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9061 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9121 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     9181 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     9241 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     9301 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     9361 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     9421 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9601 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     9661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     9721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     9901 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    10021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    10321 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    10381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10621 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10681 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    10741 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
    10801 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
    10861 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
    10921 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    10981 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    11041 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    11101 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    11161 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    11221 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    11281 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    11341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    11461 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    11521 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11581 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    11641 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    11701 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    11761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    11821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    11881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    12001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    12061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    12121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    12241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    12301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    12361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    12421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    12481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    12541 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    12601 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    12661 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    12721 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    12781 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    12841 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    12901 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    12961 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    13021 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    13081 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    13141 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    13201 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    13261 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    13321 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    13381 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    13441 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    13501 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    13561 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    13621 gaaaagtgcc acctgac
//
