LOCUS       V014992                13901 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014992
VERSION     V014992
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        266..341
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        403..614
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             639..4742
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4791..4814
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4824..4880
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             4881..6527
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             6528..6581
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             6582..7178
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7204..7792
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7864..8097
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8129..8353
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8399..8827
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8970..9103
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(9188..9218)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9233..9254)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9542..10130)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10304..11161)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11162..11266)
                     /label="AmpR promoter"
     enhancer        11532..11911
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11912..12114
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             12129..12309
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12356..12481
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12974..13207
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             13392..13436
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             13585..13626
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    13734..13851
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggacg tctctgtttt agagctagaa atagcaagtt aaaataaggc
      301 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttcgctagct
      361 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
      421 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
      481 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      541 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      601 ccgccagaac acaggaccgg ttctagagcg ctgccaccat ggacaagaag tacagcatcg
      661 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
      721 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
      781 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
      841 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
      901 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
      961 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     1021 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     1081 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     1141 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     1201 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     1261 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     1321 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     1381 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     1441 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1501 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1561 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1621 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1681 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     1741 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     1801 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     1861 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     1921 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     1981 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     2041 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     2101 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     2161 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     2221 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     2281 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     2341 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     2401 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     2461 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2521 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2581 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2641 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     2701 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     2761 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     2821 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     2881 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     2941 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     3001 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     3061 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     3121 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     3181 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     3241 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     3301 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     3361 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     3421 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3481 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3541 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3601 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3661 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     3721 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     3781 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     3841 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     3901 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     3961 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     4021 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     4081 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     4141 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     4201 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     4261 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     4321 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     4381 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     4441 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4501 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4561 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4621 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4681 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     4741 acaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa gattacaaag
     4801 acgatgacga taagggatcc ggcgccacca acttcagcct gctgaagcag gccggcgacg
     4861 tggaggagaa ccccggcccc gaagatgcca aaaacattaa gaagggccca gcgccattct
     4921 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     4981 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     5041 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     5101 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     5161 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     5221 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     5281 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     5341 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     5401 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     5461 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     5521 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     5581 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     5641 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     5701 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     5761 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     5821 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     5881 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     5941 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     6001 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     6061 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     6121 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     6181 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     6241 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     6301 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     6361 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     6421 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     6481 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtggag ggcagaggaa
     6541 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca
     6601 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6661 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6721 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6781 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6841 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6901 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6961 ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7021 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7081 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7141 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg actcgagtac gcgttaagtc
     7201 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7561 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7741 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     7801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7861 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     8101 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8161 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8221 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8281 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8341 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     8401 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8461 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8521 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8581 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8641 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8701 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8761 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8821 gcgaattaat tctgtggaat cacgtgctac gagatttcga ttccaccgcc gccttctatg
     8881 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8941 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9121 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9181 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9241 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9301 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9361 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9421 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9541 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9661 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9721 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9961 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10141 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10201 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10261 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10321 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10381 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10441 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10501 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10561 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10621 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10681 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10741 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10801 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10861 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10921 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10981 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11041 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11101 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11161 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11221 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11281 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    11341 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    11401 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    11461 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    11521 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    11581 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    11641 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11701 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11761 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11821 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11881 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    11941 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    12001 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    12061 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
    12121 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    12181 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    12241 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    12301 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    12361 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    12421 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    12481 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    12541 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    12601 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    12661 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    12721 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    12781 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    12841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    12901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    12961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    13021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    13081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    13141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    13201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    13261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    13321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    13381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    13441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    13501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    13561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    13621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    13681 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
    13741 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    13801 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    13861 tacagggaca gcagagatcc agtttggtta attaaggtac c
//
