LOCUS       V014850                16388 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014850
VERSION     V014850
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..16388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2608..2848
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        4738..4813
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        4875..5086
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             5111..9214
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             9263..9286
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9293..9349
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9350..10996
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             11057..11452
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    11480..12068
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             12140..12373
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12405..12629
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12675..13103
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        13117..13446
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        13494..13541
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             13560..13931
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    14064..14197
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(14282..14312)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(14327..14348)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(14636..15224)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(15398..16255)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(16256..16360)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
     5101 cgctgccacc atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg
     5161 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     5221 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     5281 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     5341 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     5401 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     5461 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     5521 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     5581 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     5641 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     5701 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     5761 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     5821 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     5881 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     5941 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     6001 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     6061 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     6121 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     6181 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     6241 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     6301 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     6361 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     6421 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     6481 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     6541 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     6601 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     6661 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     6721 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     6781 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     6841 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     6901 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     6961 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     7021 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     7081 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     7141 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     7201 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     7261 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     7321 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     7381 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     7441 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     7501 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     7561 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     7621 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     7681 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     7741 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     7801 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     7861 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     7921 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     7981 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     8041 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     8101 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     8161 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     8221 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     8281 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     8341 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     8401 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     8461 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     8521 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     8581 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     8641 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     8701 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     8761 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     8821 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     8881 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     8941 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     9001 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     9061 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     9121 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     9181 gacacggatc gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc
     9241 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccgccaccaa
     9301 cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac cccggccccg aagatgccaa
     9361 aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca
     9421 gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc
     9481 acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga
     9541 agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag
     9601 cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc
     9661 taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt
     9721 cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat
     9781 catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta
     9841 caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag
     9901 cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc
     9961 caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc
    10021 catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca
    10081 cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat
    10141 gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc
    10201 cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga
    10261 cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga
    10321 ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac
    10381 aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt
    10441 ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa
    10501 ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc
    10561 cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta
    10621 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
    10681 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
    10741 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
    10801 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
    10861 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
    10921 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
    10981 cggcaagatc gccgtgggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
    11041 agagaatcct ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
    11101 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
    11161 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
    11221 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
    11281 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
    11341 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
    11401 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagaatt
    11461 cgatatcaag cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg
    11521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    11581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    11641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    11701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    11761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    11821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    11881 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    11941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    12001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    12061 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    12121 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
    12181 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    12241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    12301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    12361 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    12421 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    12481 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    12541 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    12601 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    12661 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    12721 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    12781 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    12841 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    12901 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    12961 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    13021 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    13081 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
    13141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    13201 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    13261 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    13321 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    13381 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    13441 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
    13501 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
    13561 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
    13621 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
    13681 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
    13741 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
    13801 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
    13861 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
    13921 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    13981 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    14041 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    14101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    14161 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    14221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    14281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    14341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    14401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    14461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    14521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    14581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    14641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    14701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    14761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    14821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    14881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    14941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    15001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    15061 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    15121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    15181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    15241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    15301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    15361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    15421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    15481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    15541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    15601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    15661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    15721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    15781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    15841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    15901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    15961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    16021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    16081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    16141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    16201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    16261 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    16321 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    16381 cacctgac
//
