LOCUS       V015003                13194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015003
VERSION     V015003
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        489..564
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        626..837
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             862..4965
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             5014..5037
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5104..5820
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    6497..7085
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7157..7390
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7422..7646
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7692..8120
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8263..8396
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8481..8511)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8526..8547)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8835..9423)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9597..10454)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10455..10559)
                     /label="AmpR promoter"
     enhancer        10825..11204
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11205..11407
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             11422..11602
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11649..11774
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12267..12500
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12685..12729
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             12878..12919
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    13027..13144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggatt cattctgcat ggttgtgctt accagaccgg agatatcatc
      301 atatatgcct tgagcaactg atagctgtcg ctgtcaactg taatacgctg cttcatagca
      361 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
      421 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcct aactcaaaat
      481 ccgtctctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      541 gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta gctaggtctt gaaaggagtg
      601 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      661 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
      721 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      781 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac
      841 cggttctaga gcgctgccac catggacaag aagtacagca tcggcctgga catcggcacc
      901 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      961 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     1021 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     1081 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     1141 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     1201 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     1261 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     1321 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     1381 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     1441 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     1501 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     1561 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     1621 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     1681 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     1741 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     1801 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     1861 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     1921 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     1981 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     2041 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     2101 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     2161 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     2221 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     2281 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     2341 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     2401 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     2461 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     2521 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     2581 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     2641 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     2701 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     2761 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     2821 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     2881 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     2941 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     3001 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     3061 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     3121 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     3181 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     3241 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     3301 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     3361 tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     3421 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     3481 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     3541 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     3601 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     3661 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     3721 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     3781 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     3841 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     3901 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     3961 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     4021 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     4081 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     4141 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     4201 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     4261 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     4321 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     4381 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     4441 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     4501 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     4561 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     4621 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     4681 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     4741 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     4801 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     4861 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     4921 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaagcg acctgccgcc
     4981 acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga
     5041 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5101 ccgatggtgt ctaagggcga agagctgttc accggggtgg tgcccatcct ggtcgagctg
     5161 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5221 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5281 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5341 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5401 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5461 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5521 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5581 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5641 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5701 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5761 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5821 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5881 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5941 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6001 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6061 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6121 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6181 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6241 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6301 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6361 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6421 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactcgag
     6481 tacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6901 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7081 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7141 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7201 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7381 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     7441 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7501 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7561 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7621 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     7681 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7741 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7801 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7861 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7921 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7981 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8041 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8101 acaaaaattt aacgcgaatt aattctgtgg aatcacgtgc tacgagattt cgattccacc
     8161 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8221 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8401 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8461 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8521 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9241 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10561 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10621 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10681 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10741 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    10801 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
    10861 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    10921 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    10981 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    11041 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    11101 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    11161 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    11221 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    11281 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    11341 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    11401 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    11461 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    11521 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    11581 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    11641 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    11701 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    11761 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    11821 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    11881 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    11941 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    12001 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    12061 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    12121 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    12181 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    12241 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    12301 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    12361 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    12421 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    12481 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    12541 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    12601 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    12661 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    12721 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    12781 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    12841 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    12901 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    12961 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
    13021 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    13081 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    13141 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagg tacc
//
