LOCUS       V015005                12993 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015005
VERSION     V015005
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        288..363
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        425..636
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             661..4764
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4813..4836
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4903..5619
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    6296..6884
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6956..7189
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7221..7445
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7491..7919
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8062..8195
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8280..8310)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8325..8346)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8634..9222)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9396..10253)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10254..10358)
                     /label="AmpR promoter"
     enhancer        10624..11003
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11004..11206
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             11221..11401
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11448..11573
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12066..12299
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12484..12528
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             12677..12718
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    12826..12943
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggaat gcagggaatt cctgatcgga cgtctctgtt ttagagctag
      301 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      361 tgcttttttg aatttgctag ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
      421 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      481 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      541 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      601 gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag cgctgccacc
      661 atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      721 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      781 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      841 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      901 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      961 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1021 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1081 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1141 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1201 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1261 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1321 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1381 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1441 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1501 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1561 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1621 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1681 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1741 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     1801 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     1861 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     1921 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     1981 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     2041 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     2101 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     2161 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2221 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2281 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2341 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2401 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2461 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2521 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2581 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2641 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2701 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     2761 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     2821 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     2881 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     2941 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     3001 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     3061 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     3121 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     3181 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3241 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3301 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3361 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3421 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3481 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3541 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3601 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3661 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3721 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     3781 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     3841 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     3901 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     3961 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     4021 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     4081 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     4141 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4201 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4261 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4321 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4381 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4441 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4501 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4561 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4621 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4681 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4741 gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc tggacaggct
     4801 aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
     4861 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggtgtc taagggcgaa
     4921 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4981 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5041 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5101 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5161 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5221 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5281 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5341 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5401 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5461 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     5521 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5581 gccgccggga tcactctcgg catggacgag ctgtacaagg agggcagagg aagtcttcta
     5641 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     5701 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5761 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     5821 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5881 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5941 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6001 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga
     6061 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6121 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6181 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6241 acctggtgca tgacccgcaa gcccggtgcc tgactcgagt acgcgttaag tcgacaatca
     6301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6661 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6781 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6841 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6901 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6961 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7021 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7081 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7141 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     7201 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7261 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7321 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7381 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7441 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     7501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     7921 attctgtgga atcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8041 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8101 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8161 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10381 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    10441 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    10501 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    10561 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
    10621 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
    10681 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    10741 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
    10801 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
    10861 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
    10921 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
    10981 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
    11041 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
    11101 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
    11161 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
    11221 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    11281 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    11341 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    11401 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
    11461 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
    11521 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
    11581 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
    11641 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    11701 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    11761 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    11821 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    11881 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
    11941 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12001 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12061 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12121 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12181 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12241 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12301 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    12361 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12421 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    12481 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    12541 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    12601 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    12661 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    12721 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
    12781 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
    12841 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    12901 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
    12961 cagcagagat ccagtttggt taattaaggt acc
//
