LOCUS       V014217                13138 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014217
VERSION     V014217
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        273..348
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        410..621
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             646..4749
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4798..4821
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4888..5604
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    5623..6211
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6283..6516
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6548..6772
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6818..7246
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7260..7589
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        7637..7684
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             7703..8074
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    8207..8340
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8425..8455)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8470..8491)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8779..9367)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9541..10398)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10399..10503)
                     /label="AmpR promoter"
     enhancer        10769..11148
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11149..11351
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             11366..11546
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11593..11718
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12211..12444
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12629..12673
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             12822..12863
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    12971..13088
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccc gttgaccata ggcccgtaca gggttttaga gctagaaata gcaagttaaa
      301 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc
      361 gctagctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      421 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag
      481 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      541 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      601 gggtttgccg ccagaacaca ggaccggttc tagagcgctg ccaccatgga caagaagtac
      661 agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      721 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      781 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      841 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      901 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
      961 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     1021 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     1081 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     1141 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     1201 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     1261 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     1321 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     1381 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     1441 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     1501 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     1561 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     1621 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     1681 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     1741 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     1801 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     1861 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     1921 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     1981 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     2041 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     2101 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     2161 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     2221 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     2281 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     2341 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     2401 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     2461 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     2521 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     2581 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     2641 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     2701 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     2761 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     2821 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     2881 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     2941 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     3001 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     3061 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     3121 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     3181 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     3241 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     3301 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     3361 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     3421 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     3481 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     3541 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     3601 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     3661 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     3721 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     3781 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     3841 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     3901 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     3961 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     4021 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     4081 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     4141 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     4201 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     4261 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     4321 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     4381 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     4441 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     4501 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     4561 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     4621 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     4681 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     4741 ggaggcgaca agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaagat
     4801 tacaaagacg atgacgataa gggatccggc gcaacaaact tctctctgct gaaacaagcc
     4861 ggagatgtcg aagagaatcc tggaccgatg gtgtctaagg gcgaagagct gttcaccggg
     4921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5581 ctcggcatgg acgagctgta caagtaaacg cgttaagtcg acaatcaacc tctggattac
     5641 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5701 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5761 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5821 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5881 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5941 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6001 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6061 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6121 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6181 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     6241 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6301 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6361 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6421 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6481 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     6541 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6601 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6661 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6721 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6781 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6961 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7021 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7201 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7261 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7321 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7381 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7441 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7501 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7561 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7621 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7681 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7741 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7801 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7861 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7921 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     7981 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8041 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8221 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8281 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8341 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10501 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    10561 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    10621 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    10681 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    10741 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
    10801 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    10861 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    10921 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    10981 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    11041 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    11101 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    11161 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    11221 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    11281 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    11341 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    11401 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11461 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11521 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    11581 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    11641 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    11701 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    11761 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    11821 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    11881 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    11941 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    12001 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    12061 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    12121 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    12181 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    12241 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    12301 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    12361 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    12421 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    12481 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    12541 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    12601 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    12661 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    12721 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    12781 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    12841 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
    12901 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
    12961 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    13021 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    13081 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt aaggtacc
//
