LOCUS       V014601                 8286 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014601
VERSION     V014601
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(2056..2085)
                     /label="NFAT binding site"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        2093..2206
                     /label="IL-2 promoter"
                     /note="minimal promoter from the human interleukin-2 gene"
     CDS             2254..3903
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             3916..3969
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3976..4371
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    4381..4969
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5043..5276
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5348..5482
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5488..5623
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5661..5677)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5685..5701)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5709..5739)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5754..5775)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6063..6651)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6825..7682)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7683..7787)
                     /label="AmpR promoter"
     primer_bind     8261..8277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtaaggatc tgcgatcgct ccggtgcccg tcagtacgcc ttctgtatga
     1981 aacagttttt cctccacgcc ttctgtatga aacagttttt cctccacgcc ttctgtatga
     2041 aacagttttt cctccacgcc ttctgtatga aacagttttt cctcctcgag gacattttga
     2101 cacccccata atatttttcc agaattaaca gtataaattg catctcttgt tcaagagttc
     2161 cctatcactc tctttaatca ctactcacag taacctcaac tcctgcccaa gcttcaagct
     2221 gtgaccggcg cctactctag agctagcgaa ttcatggaag atgccaaaaa cattaagaag
     2281 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     2341 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     2401 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     2461 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     2521 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     2581 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     2641 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     2701 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     2761 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     2821 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     2881 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     2941 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     3001 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     3061 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     3121 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     3181 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     3241 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     3301 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     3361 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     3421 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     3481 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     3541 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     3601 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     3661 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     3721 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     3781 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     3841 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     3901 gtgttagcgg ccgctgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     3961 cccggccctt ccggaatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     4021 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     4081 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     4141 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     4201 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     4261 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     4321 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagtcgac
     4381 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4441 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4501 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4561 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4621 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4681 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4741 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4801 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4861 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4921 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     4981 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5041 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     5101 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5161 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5221 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5281 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5341 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5461 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     5521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     5641 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7801 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7861 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8041 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8101 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8161 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8221 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     8281 aagctg
//
