LOCUS       .                       8806 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2032..2063
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2112..3761
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        3776..3987
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             4000..4268
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4295..4891
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4901..5489
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5563..5796
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5868..6002
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6008..6143
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6181..6197)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6205..6221)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6229..6259)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6274..6295)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6583..7171)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7345..8202)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8203..8307)
                     /label="AmpR promoter"
     primer_bind     8781..8797
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtaaggatct gcgatcgctc cggtgcccgt cagtgaaact gaaactgaaa
     1981 ctgaaactga aactgaaact gaaactgaaa ctgaaactga aacttcgagg atagagggta
     2041 tataatggaa gctcgaattc cagccaagca ttcaagctgt gaccggcgcc tactctagag
     2101 ctagcgaatt catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
     2161 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
     2221 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
     2281 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
     2341 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
     2401 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
     2461 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
     2521 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
     2581 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
     2641 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     2701 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     2761 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     2821 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     2881 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     2941 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     3001 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     3061 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     3121 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     3181 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     3241 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     3301 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     3361 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     3421 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     3481 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     3541 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     3601 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     3661 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     3721 agattctcat taaggccaag aagggcggca agatcgccgt gtgattagcg gccgcgggca
     3781 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     3841 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     3901 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     3961 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4021 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4081 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4141 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4201 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     4261 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg
     4321 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     4381 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     4441 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4501 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4561 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4621 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4681 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4741 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4801 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4861 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa
     4921 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4981 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5041 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5101 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5161 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5221 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5281 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5341 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5401 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5461 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     5521 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5581 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5821 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6001 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6121 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     6181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8401 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8461 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8521 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     8581 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     8641 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     8701 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     8761 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
