LOCUS       .                       8064 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1933..2147
                     /label="H1 promoter"
                     /note="human H1 RNA promoter"
     promoter        2223..2434
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             2447..2715
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2771..3436
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             3506..3559
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3560..4156
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4160..4747
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4821..5054
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5126..5260
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5266..5401
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5439..5455)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5463..5479)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5487..5517)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5532..5553)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5841..6429)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6603..7460)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7461..7565)
                     /label="AmpR promoter"
     primer_bind     8039..8055
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc
     1981 actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg
     2041 ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt
     2101 gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccactca tcgctagcga
     2161 attcgaattt aaatcggatc cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     2221 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2281 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2341 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2401 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     2461 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     2521 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     2581 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     2641 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     2701 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga
     2761 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     2821 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     2881 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     2941 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     3001 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     3061 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     3121 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     3181 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     3241 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     3301 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     3361 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     3421 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     3481 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt
     3541 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3601 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3661 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3721 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3781 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3841 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3901 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3961 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4021 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4081 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4141 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4201 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4261 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4321 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4381 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4441 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4501 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4561 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4621 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4681 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc
     4741 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     4861 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4921 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4981 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5041 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5101 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     5161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5221 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     5281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5341 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5401 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     5461 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5521 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5581 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5641 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6001 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6061 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6121 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6181 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6241 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6301 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6361 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6421 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6481 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6541 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6601 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6661 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6721 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6781 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6841 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6901 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6961 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7021 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7081 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7141 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7201 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7261 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7321 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7381 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7441 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7501 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7561 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7621 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     7681 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7741 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     7801 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7861 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     7921 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7981 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     8041 aaaacgacgg ccagtgccaa gctg
//
