LOCUS       V014379                15336 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014379
VERSION     V014379
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2687..3865
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3884..3904
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3914..8017
                     /label="Cas9m4"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             8072..8092
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9287..10000
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    10028..10616
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             11141..11321
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11353..11577
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11623..12051
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        12065..12394
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        12442..12489
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             12508..12879
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    13012..13145
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(13230..13260)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(13275..13296)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13584..14172)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(14346..15203)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(15204..15308)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg agccccaaga agaagagaaa
     3901 ggtggaggcc agcatggaca agaagtactc cattgggctc gctatcggca caaacagcgt
     3961 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     4021 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     4081 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     4141 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     4201 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     4261 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     4321 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     4381 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     4441 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     4501 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     4561 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     4621 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     4681 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
     4741 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
     4801 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
     4861 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
     4921 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
     4981 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
     5041 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
     5101 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
     5161 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
     5221 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
     5281 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg
     5341 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
     5401 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
     5461 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
     5521 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
     5581 ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga
     5641 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
     5701 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
     5761 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
     5821 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
     5881 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
     5941 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
     6001 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
     6061 gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
     6121 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
     6181 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
     6241 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
     6301 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
     6361 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
     6421 cgacgtggct gctatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
     6481 gttgacaaga tccgataaag ctagagggaa gagtgataac gtcccctcag aagaagttgt
     6541 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
     6601 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
     6661 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
     6721 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
     6781 tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag
     6841 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
     6901 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
     6961 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
     7021 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
     7081 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
     7141 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
     7201 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
     7261 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
     7321 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
     7381 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
     7441 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
     7501 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
     7561 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
     7621 gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca
     7681 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
     7741 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
     7801 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
     7861 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
     7921 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
     7981 tgaaacaaga atcgacctct ctcagctcgg tggagacagc gctggaggag gtggaagcgg
     8041 aggaggagga agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc
     8101 tggatccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
     8161 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
     8221 catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta acgctagcgg
     8281 cagtggacct acacaggccg gcgagggcac actgtctgaa gctctgctgc agctgcagtt
     8341 cgacgacgag gatctgggag ccctgctggg aaacagcacc gatcctgccg tgttcaccga
     8401 cctggccagc gtggacaaca gcgagttcca gcagctgctg aaccagggca tccctgtggc
     8461 ccctcacacc accgagccca tgctgatgga ataccccgag gccatcaccc ggctcgtgac
     8521 aggcgctcag aggcctcctg atccagctcc tgcccctctg ggagcaccag gcctgcctaa
     8581 tggactgctg tctggcgacg aggacttcag ctctatcgcc gatatggatt tctcagcctt
     8641 gctgggctct ggcagcggca gccgggattc cagggaaggg atgtttttgc cgaagcctga
     8701 ggccggctcc gctattagtg acgtgtttga gggccgcgag gtgtgccagc caaaacgaat
     8761 ccggccattt catcctccag gaagtccatg ggccaaccgc ccactccccg ccagcctcgc
     8821 accaacacca accggtccag tacatgagcc agtcgggtca ctgaccccgg caccagtccc
     8881 tcagccactg gatccagcgc ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc
     8941 cgatgaagag acgagccagg ctgtcaaagc ccttcgggag atggccgata ctgtgattcc
     9001 ccagaaggaa gaggctgcaa tctgtggcca aatggacctt tcccatccgc ccccaagggg
     9061 ccatctggat gagctgacaa ccacacttga gtccatgacc gaggatctga acctggactc
     9121 acccctgacc ccggaattga acgagattct ggataccttc ctgaacgacg agtgcctctt
     9181 gcatgccatg catatcagca caggactgtc catcttcgac acatctctct ttgagggcag
     9241 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga gcaagggcga
     9301 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9361 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9421 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     9481 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9541 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9601 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9661 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     9721 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     9781 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     9841 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     9901 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     9961 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattcg atatcaagct
    10021 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    10081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    10141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    10201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    10261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    10321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    10381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    10441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    10501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    10561 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    10621 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
    10681 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
    10741 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    10801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
    10861 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
    10921 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
    10981 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
    11041 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
    11101 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
    11161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11281 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
    11341 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    11401 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    11461 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    11521 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    11581 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    11641 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    11701 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    11761 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    11821 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    11881 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    11941 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    12001 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    12061 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    12121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    12181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    12241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    12301 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    12361 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    12421 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    12481 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    12541 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    12601 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    12661 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    12721 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    12781 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    12841 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    12901 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    12961 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    13021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    13081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    13141 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    13201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    13261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    13321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    13381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    13441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    13501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    13561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    13621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    13681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    13741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    13801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    13861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    13921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    13981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    14041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    14101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    14161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    14221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    14281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    14341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    14401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    14461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    14521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    14581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    14641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    14701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    14761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    14821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    14881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    14941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    15001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    15061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    15121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    15181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    15241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    15301 gttccgcgca catttccccg aaaagtgcca cctgac
//
