LOCUS       .                      10135 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1960..2171
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             2184..2452
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4049..4078
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     promoter        4294..4793
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4835..5500
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             5570..5623
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             5624..6220
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6230..6818
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6892..7125
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7197..7331
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7337..7472
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7558..7588)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7603..7624)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7912..8500)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8674..9531)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9532..9636)
                     /label="AmpR promoter"
     primer_bind     10110..10126
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctctgcctg tgactgccct
     2521 gctgctgcca ctggccctgc tgctgcatgc cgccaggcct gaggtgcagc tggtggagag
     2581 cggtggcgga ctggtccagc ctggcgagtc tctgcggctg tcttgtgctg cctctggatt
     2641 cacattcgat acctacgcca tgcactgggt gagacaggcc cctggaaaat gcctggagtg
     2701 ggtggccggc atctcctgga actccgggaa tattggctac gccgattctg tgaaaggccg
     2761 gttcacaatc tccagagaca acagtaagaa taccctgtac ctgcagatga actctctgag
     2821 agccgaggac acagcagtct actactgcgc caaggattgg gacaattgga atctgttcga
     2881 ctactggggc caggggaccc tggtgacagt gtccagcggc ggaggcggga gcggcggggg
     2941 cggcagcggc ggaggcggaa gcgacattca gatgacacaa agcccctcat ccctgagcgc
     3001 atctgtgggc gacagagtga ctattacctg cagagcttcc cagtccattt ctagctggct
     3061 ggcctggtat cagcagaagc caggaaaggc tccaaagctg ctgatctaca aagccagcag
     3121 cctggagtcc ggagtgcctt cacgcttcag cggcagcggg tcaggcaccg acttcaccct
     3181 gacaatttcc agcctgcagc cagaggactt tgccacctac tactgccagc agtacagttc
     3241 ttacagctgg acctttggtt gtggcaccaa ggtggagatt aagagaggag gcggaggtag
     3301 tggaggaggg ggaagcggag gcggagggag tgaggtgcag ctgctggaga gcggtggcgg
     3361 ccttgtgcag ccaggcgggt ctctgaggct gtcctgcgcc gccagcggct ttacattctc
     3421 ctcttacgct atgagctggg tccggcaggc cccaggcaaa ggccttgagt gggtgagtgc
     3481 catcagtggc agcggcggca gcacatacta cgctgactcc gtgaagggaa gatttaccat
     3541 tagcagagat aacagcaaaa acaccctgta cctgcagatg aacagcctgc gagccgaaga
     3601 caccgccgtg tactactgcg caaaatccta cggcgctttc gactattggg gccaggggac
     3661 cctggtgacc gtgagcagcg gtggaggcgg atcaggaggc ggtggatctg gcggtggagg
     3721 ttcagatatc cagatgaccc agagccccag ctctctgagt gcctccgtgg gggacagagt
     3781 gaccatcaca tgtcaggcct ctcaggacat caataagtac ctgaactggt atcagcagaa
     3841 gcccggtaag gccccaaaac tgctgattta tggcgccagc agactggaga caggcgtgcc
     3901 cagcagattc agcggatcag gaagcggaac ggactttaca tttaccatct ccagcctgca
     3961 gcccgaggac atcgccactt actactgcca gcagaaacat ccaaggggcc caagaacctt
     4021 cggccaggga acaaaagtgg aaatcaagga acaaaaactc atctcagaag aggatctgat
     4081 cagcaacagc gtgatgtact tcagcagcgt ggtgcccgtg ctgcagaaag tgaacagcac
     4141 caccaccaag cctgtgctga gaacccccag ccctgtgcac cctaccggca gcagcctgaa
     4201 gggcaccggc atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc
     4261 actggttatc accctttact gctagcggcc gctgggtagg ggaggcgctt ttcccaaggc
     4321 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4381 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4441 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4501 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4561 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4621 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4681 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4741 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     4801 gcccaagctt accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
     4861 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
     4921 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
     4981 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
     5041 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
     5101 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
     5161 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
     5221 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
     5281 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
     5341 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
     5401 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
     5461 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc
     5521 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg
     5581 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc
     5641 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5701 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5761 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5821 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5881 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5941 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6001 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6061 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6121 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6181 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     6241 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6301 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6361 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6421 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6481 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6541 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6601 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6661 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6721 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6781 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     6841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6901 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     6961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7141 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7201 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7321 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7441 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     7501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8341 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9661 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9721 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9781 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9841 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     9901 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9961 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10021 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10081 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
