LOCUS       .                       8932 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1960..2171
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             2184..2452
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4301..5017
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    5027..5615
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5689..5922
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5994..6128
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6134..6269
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6307..6323)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6331..6347)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6355..6385)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6400..6421)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6709..7297)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7471..8328)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8329..8433)
                     /label="AmpR promoter"
     primer_bind     8907..8923
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctctgcctg tgactgccct
     2521 gctgctgcca ctggccctgc tgctgcatgc cgccaggcct gaggtgcagc tggtggagag
     2581 cggtggcgga ctggtccagc ctggcgagtc tctgcggctg tcttgtgctg cctctggatt
     2641 cacattcgat acctacgcca tgcactgggt gagacaggcc cctggaaaat gcctggagtg
     2701 ggtggccggc atctcctgga actccgggaa tattggctac gccgattctg tgaaaggccg
     2761 gttcacaatc tccagagaca acagtaagaa taccctgtac ctgcagatga actctctgag
     2821 agccgaggac acagcagtct actactgcgc caaggattgg gacaattgga atctgttcga
     2881 ctactggggc caggggaccc tggtgacagt gtccagcggc ggaggcggga gcggcggggg
     2941 cggcagcggc ggaggcggaa gcgacattca gatgacacaa agcccctcat ccctgagcgc
     3001 atctgtgggc gacagagtga ctattacctg cagagcttcc cagtccattt ctagctggct
     3061 ggcctggtat cagcagaagc caggaaaggc tccaaagctg ctgatctaca aagccagcag
     3121 cctggagtcc ggagtgcctt cacgcttcag cggcagcggg tcaggcaccg acttcaccct
     3181 gacaatttcc agcctgcagc cagaggactt tgccacctac tactgccagc agtacagttc
     3241 ttacagctgg acctttggtt gtggcaccaa ggtggagatt aagagaggag gcggaggtag
     3301 tggaggaggg ggaagcggag gcggagggag tgaggtgcag ctgctggaga gcggtggcgg
     3361 ccttgtgcag ccaggcgggt ctctgaggct gtcctgcgcc gccagcggct ttacattctc
     3421 ctcttacgct atgagctggg tccggcaggc cccaggcaaa ggccttgagt gggtgagtgc
     3481 catcagtggc agcggcggca gcacatacta cgctgactcc gtgaagggaa gatttaccat
     3541 tagcagagat aacagcaaaa acaccctgta cctgcagatg aacagcctgc gagccgaaga
     3601 caccgccgtg tactactgcg caaaatccta cggcgctttc gactattggg gccaggggac
     3661 cctggtgacc gtgagcagcg gtggaggcgg atcaggaggc ggtggatctg gcggtggagg
     3721 ttcagatatc cagatgaccc agagccccag ctctctgagt gcctccgtgg gggacagagt
     3781 gaccatcaca tgtcaggcct ctcaggacat caataagtac ctgaactggt atcagcagaa
     3841 gcccggtaag gccccaaaac tgctgattta tggcgccagc agactggaga caggcgtgcc
     3901 cagcagattc agcggatcag gaagcggaac ggactttaca tttaccatct ccagcctgca
     3961 gcccgaggac atcgccactt actactgcca gcagaaacat ccaaggggcc caagaacctt
     4021 cggccaggga acaaaagtgg aaatcaagga acaaaaactc atctcagaag aggatctgat
     4081 cagcaacagc gtgatgtact tcagcagcgt ggtgcccgtg ctgcagaaag tgaacagcac
     4141 caccaccaag cctgtgctga gaacccccag ccctgtgcac cctaccggca gcagcctgaa
     4201 gggcaccggc atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc
     4261 actggttatc accctttact gcggatccgc ggccgccacc atggtgagca agggcgagga
     4321 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4381 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4441 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4501 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4561 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4621 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4681 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4741 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4801 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4861 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4921 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4981 cgccgggatc actctcggca tggacgagct gtacaagtaa gtcgacaatc aacctctgga
     5041 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5101 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5161 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5221 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5281 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5341 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5401 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5461 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5521 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5581 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     5641 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5701 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5941 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6121 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6181 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6241 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     6301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8461 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8521 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     8581 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     8641 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     8701 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8761 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8821 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8881 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
