LOCUS       V022607                10588 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022607
VERSION     V022607
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2498..4729
                     /label="Slc26a5(NM_030727)"
                     /note="Slc26a5(NM_030727)"
                     /gene="Slc26a5"
     promoter        4747..5246
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5291..5953
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6023..6076
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6077..6676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6683..7271
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7345..7578
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7650..7771
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7790..7925
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7963..7979)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7987..8003
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8011..8041)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8056..8077
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8365..8953)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9124..9984)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9985..10089)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10563..10579
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gatcatgctg aagaaactga
     2521 aatccctgca gagacccaga ggtactacgt ggaaaggccc atcttcagtc atcctgtcct
     2581 ccaagagagg ctgcacgtca aggacaaagt cacagagtcc attggagata agctgaagca
     2641 ggcattcacg tgtactccta aaaaaataag aaacatcatt tacatgttcc tgcctatcac
     2701 taagtggctg ccagcatata aattcaagga gtatgtgtta ggtgacttgg tctcgggcat
     2761 aagcactggg gtactccagc ttccccaagg cttagccttc gccatgctgg cagccgtgcc
     2821 tccggtgttt ggcctgtact catcgtttta ccccgttatc atgtactgtt tctttggaac
     2881 ctcaagacac atatctatag gtccttttgc tgttattagc ttgatgattg gaggtgtggc
     2941 cgtccggtta gtaccagatg atattgtcat cccaggagga gtaaatgcaa ccaacgggac
     3001 agaagccaga gatgcactaa gagtgaaagt cgccatgtct gttaccttac tttcaggaat
     3061 cattcagttt tgcctaggtg tctgtaggtt tggatttgtg gccatatacc tcacggagcc
     3121 attggtgcga ggctttacca ctgcggctgc tgtccacgtg ttcacgtcca tgttaaaata
     3181 cctgtttggg gtcaaaacaa agcggtacag tggaatcttt tcagtggtgt atagtacagt
     3241 tgctgtgttg cagaatgtta aaaacctcaa cgtgtgttcc ctaggcgtcg gcctgatggt
     3301 ttttggtttg ctgttgggtg gcaaggaatt taatgagaga tttaaagaga aattgccagc
     3361 acccattcct ctagagttct ttgctgtggt gatggggact ggcatttctg caggatttag
     3421 cctacatgag tcctacagtg tggatgtcgt tggaacactt cctctggggc tacttcctcc
     3481 ggccaaccca gacaccagcc tgttccacct ggtgtatgtg gacgccattg ccatcgccat
     3541 cgttggattt tcagtgacga tctccatggc caaaaccttg gcaaataagc atggctacca
     3601 ggttgatggc aatcaggagc tcattgcctt ggggatatgc aactccattg gatctctctt
     3661 ccaaaccttc tcgatttcct gctccttgtc tcgaagcctt gttcaggaag gaactggagg
     3721 gaaaacacag cttgcaggtt gtttggcctc gttgatgatt ctgttggtca tattagccac
     3781 cggattcctc tttgagtcgt taccccaggc tgtcctttcc gccattgtga tcgtcaacct
     3841 gaaaggaatg ttcatgcagt tctcagacct gccttttttc tggagaacca gcaaaataga
     3901 gctgaccatc tggctgacca cctttgtgtc ctccctgttc ctcggcttgg actacggact
     3961 gattaccgcc gtgatcattg ctctgctcac agtgatttat agaacacaga gtccaagcta
     4021 caaagtcctg gggcagctcc ctgacacgga tgtgtacatt gacatagatg catatgagga
     4081 ggtgaaagaa attcctggaa taaaaatatt ccaaataaat gccccaattt actatgcaaa
     4141 tagcgacttg tatagcagcg ctttaaaaag aaagactgga gtaaacccag cactcattat
     4201 gggagcgaga agaaaggcca tgaggaagta cgccaaggaa gttggaaatg ccaacgtggc
     4261 caatgctact gttgtcaaag tggatgcaga agtagacgga gaaaatgcta caaaacctga
     4321 agaagaggat gatgaagtca aatttccccc aatagtcatc aaaacaacat ttcctgaaga
     4381 gctgcagaga tttttgcccc agggggaaaa tgtccacact gtcattctag actttacgca
     4441 ggtcaatttt gtggattctg ttggagtgaa aactctggcc gggattgtga aagaatatgg
     4501 agatgttgga atttatgtat atttagcagg atgcagccca caagttgtga atgacctcac
     4561 ccgcaacaac ttttttgaaa atcctgcctt gaaagagctt ctgttccaca gtatccacga
     4621 tgcagtcctg ggcagccaag ttcgggaggc aatggctgaa caagaagcca cagcgtcact
     4681 tccccaggag gatatggagc ccaatgccac acccaccacc cccgaggcat aggatccgcg
     4741 gccgctgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4801 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4861 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4921 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4981 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5041 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5101 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5161 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5221 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatgg agagcgacga
     5281 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     5341 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     5401 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     5461 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     5521 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     5581 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     5641 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     5701 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     5761 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     5821 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     5881 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     5941 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     6001 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     6061 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6661 caagcccggt gcctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6721 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6781 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6841 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6901 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6961 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7021 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7081 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7141 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7201 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7261 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7321 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc
     7381 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7441 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7501 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7561 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7621 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7681 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7921 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt
     7981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8161 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9001 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9061 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9121 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9181 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9241 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9301 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9361 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9421 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9481 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9541 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9601 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9661 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9721 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9781 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9841 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9901 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9961 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10021 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10081 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10141 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10201 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10261 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10321 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10381 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10441 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10501 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10561 ttgtaaaacg acggccagtg ccaagctg
//
