LOCUS       V014509                10475 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014509
VERSION     V014509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1960..2171
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             2184..2452
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2498..4621
                     /label="large T antigen"
                     /note="large T antigen from SV40 (simian virus 40)"
     promoter        4634..5133
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5175..5840
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             5910..5963
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             5964..6560
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6570..7158
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7232..7465
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7537..7671
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7677..7812
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7898..7928)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7943..7964)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8252..8840)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9014..9871)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9872..9976)
                     /label="AmpR promoter"
     primer_bind     10450..10466
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gataaagttt taaacagaga
     2521 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgcctggg ggaatattcc
     2581 tctgatgaga aaggcatatt taaaaaaatg caaggagttt catcctgata aaggaggaga
     2641 tgaagaaaaa atgaagaaaa tgaatactct gtacaagaaa atggaagatg gagtaaaata
     2701 tgctcatcaa cctgactttg gaggcttctg ggatgcaact gagattccaa cctatggaac
     2761 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     2821 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     2881 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     2941 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     3001 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     3061 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     3121 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     3181 ggaatatttg atgtatagtg ccttgactag agatccattt tctgttattg aggaaagttt
     3241 gccaggtggg ttaaaggagc atgattttaa tccagaagaa gcagaggaaa ctaaacaagt
     3301 gtcctggaag cttgtaacag agtatgcaat ggaaacaaaa tgtgatgatg tgttgttatt
     3361 gcttgggatg tacttggaat ttcagtacag ttttgaaatg tgtttaaaat gtattaaaaa
     3421 agaacagccc agccactata agtaccatga aaagcattat gcaaatgctg ctatatttgc
     3481 tgacagcaaa aaccaaaaaa ccatatgcca acaggctgtt gatactgttt tagctaaaaa
     3541 gcgggttgat agcctacaat taactagaga acaaatgtta acaaacagat ttaatgatct
     3601 tttggatagg atggatataa tgtttggttc tacaggctct gctgacatag aagaatggat
     3661 ggctggagtt gcttggctac actgtttgtt gcccaaaatg gattcagtgg tgtatgactt
     3721 tttaaaatgc atggtgtaca acattcctaa aaaaagatac tggctgttta aaggaccaat
     3781 tgatagtggt aaaactacat tagcagctgc tttgcttgaa ttatgtgggg ggaaagcttt
     3841 aaatgttaat ttgcccttgg acaggctgaa ctttgagcta ggagtagcta ttgaccagtt
     3901 tttagtagtt tttgaggatg taaagggcac tggaggggag tccagagatt tgccttcagg
     3961 tcagggaatt aataacctgg acaatttaag ggattatttg gatggcagtg ttaaggtaaa
     4021 cttagaaaag aaacacctaa ataaaagaac tcaaatattt ccccctggaa tagtcaccat
     4081 gaatgagtac agtgtgccta aaacactgca ggccagattt gtaaaacaaa tagattttag
     4141 gcccaaagat tatttaaagc attgcctgga acgcagtgag tttttgttag aaaagagaat
     4201 aattcaaagt ggcattgctt tgcttcttat gttaatttgg tacagacctg tggctgagtt
     4261 tgctcaaagt attcagagca gaattgtgga gtggaaagag agattggaca aagagtttag
     4321 tttgtcagtg tatcaaaaaa tgaagtttaa tgtggctatg ggaattggag ttttagattg
     4381 gctaagaaac agtgatgatg atgatgaaga cagccaggaa aatgctgata aaaatgaaga
     4441 tggtggggag aagaacatgg aagactcagg gcatgaaaca ggcattgatt cacagtccca
     4501 aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca
     4561 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4621 ataagcggcc gctgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4681 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     4741 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     4801 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     4861 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     4921 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     4981 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     5041 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     5101 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatggaga
     5161 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     5221 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     5281 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     5341 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     5401 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     5461 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     5521 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     5581 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     5641 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     5701 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     5761 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     5821 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     5881 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg
     5941 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6001 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6061 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6121 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6181 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6241 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6301 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6361 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6421 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6481 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6541 tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6601 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6661 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6721 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6781 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6841 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6901 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6961 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7021 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7081 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7141 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7201 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7261 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7441 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7501 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7681 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7741 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7801 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     7861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10021 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10081 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10141 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10201 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10261 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10321 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10381 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10441 cacgacgttg taaaacgacg gccagtgcca agctg
//
