LOCUS       V014716                10552 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014716
VERSION     V014716
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1960..2171
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             2184..2452
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2498..4621
                     /label="large T antigen"
                     /note="large T antigen from SV40 (simian virus 40)"
     enhancer        4634..5013
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5014..5217
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5243..5261
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5303..5968
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             5978..6034
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             6041..6637
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6647..7235
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7309..7542
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7614..7748
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7754..7889
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7975..8005)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8020..8041)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8329..8917)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9091..9948)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9949..10053)
                     /label="AmpR promoter"
     primer_bind     10527..10543
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gataaagttt taaacagaga
     2521 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgcctggg ggaatattcc
     2581 tctgatgaga aaggcatatt taaaaaaatg caaggagttt catcctgata aaggaggaga
     2641 tgaagaaaaa atgaagaaaa tgaatactct gtacaagaaa atggaagatg gagtaaaata
     2701 tgctcatcaa cctgactttg gaggcttctg ggatgcaact gagattccaa cctatggaac
     2761 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     2821 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     2881 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     2941 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     3001 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     3061 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     3121 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     3181 ggaatatttg atgtatagtg ccttgactag agatccattt tctgttattg aggaaagttt
     3241 gccaggtggg ttaaaggagc atgattttaa tccagaagaa gcagaggaaa ctaaacaagt
     3301 gtcctggaag cttgtaacag agtatgcaat ggaaacaaaa tgtgatgatg tgttgttatt
     3361 gcttgggatg tacttggaat ttcagtacag ttttgaaatg tgtttaaaat gtattaaaaa
     3421 agaacagccc agccactata agtaccatga aaagcattat gcaaatgctg ctatatttgc
     3481 tgacagcaaa aaccaaaaaa ccatatgcca acaggctgtt gatactgttt tagctaaaaa
     3541 gcgggttgat agcctacaat taactagaga acaaatgtta acaaacagat ttaatgatct
     3601 tttggatagg atggatataa tgtttggttc tacaggctct gctgacatag aagaatggat
     3661 ggctggagtt gcttggctac actgtttgtt gcccaaaatg gattcagtgg tgtatgactt
     3721 tttaaaatgc atggtgtaca acattcctaa aaaaagatac tggctgttta aaggaccaat
     3781 tgatagtggt aaaactacat tagcagctgc tttgcttgaa ttatgtgggg ggaaagcttt
     3841 aaatgttaat ttgcccttgg acaggctgaa ctttgagcta ggagtagcta ttgaccagtt
     3901 tttagtagtt tttgaggatg taaagggcac tggaggggag tccagagatt tgccttcagg
     3961 tcagggaatt aataacctgg acaatttaag ggattatttg gatggcagtg ttaaggtaaa
     4021 cttagaaaag aaacacctaa ataaaagaac tcaaatattt ccccctggaa tagtcaccat
     4081 gaatgagtac agtgtgccta aaacactgca ggccagattt gtaaaacaaa tagattttag
     4141 gcccaaagat tatttaaagc attgcctgga acgcagtgag tttttgttag aaaagagaat
     4201 aattcaaagt ggcattgctt tgcttcttat gttaatttgg tacagacctg tggctgagtt
     4261 tgctcaaagt attcagagca gaattgtgga gtggaaagag agattggaca aagagtttag
     4321 tttgtcagtg tatcaaaaaa tgaagtttaa tgtggctatg ggaattggag ttttagattg
     4381 gctaagaaac agtgatgatg atgatgaaga cagccaggaa aatgctgata aaaatgaaga
     4441 tggtggggag aagaacatgg aagactcagg gcatgaaaca ggcattgatt cacagtccca
     4501 aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca
     4561 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4621 ataagcggcc gctgacattg attattgact agttattaat agtaatcaat tacggggtca
     4681 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4741 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4801 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4861 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     4921 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     4981 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     5041 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5101 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     5161 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     5221 ttagtgaacc gtcagaattt tgtaatacga ctcactatag ggcggccggg aattcgccac
     5281 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac
     5341 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg
     5401 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     5461 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     5521 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     5581 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     5641 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     5701 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     5761 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     5821 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     5881 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     5941 cgccttcaag acccccatcg ccttcgccgg ttcgtccgct actaacttca gcctgctgaa
     6001 gcaggctgga gacgtggagg agaaccctgg acctgtcgac atgaccgagt acaagcccac
     6061 ggtgcgcctc gccacccgcg acgacgtccc ccgggcagta cgcaccctcg ccgccgcgtt
     6121 cgccgactac cccgccacgc gccacaccgt cgatccagac cgccacatcg agcgggtcac
     6181 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6241 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6301 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6361 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6421 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6481 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6541 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6601 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga
     6661 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6721 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6781 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6841 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6901 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6961 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7021 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7081 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7141 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7201 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     7261 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7321 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7501 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7561 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7741 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7861 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     7921 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8521 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8761 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8881 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8941 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9001 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9061 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9121 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9181 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9241 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9301 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9361 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9421 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9481 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9541 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9601 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9661 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9721 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9781 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9841 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9901 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9961 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10021 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10081 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10141 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10201 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10261 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10321 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10381 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10441 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10501 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
