LOCUS       V014399                 9294 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014399
VERSION     V014399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3016..3039
                     /label="OVA peptide"
                     /note="antigenic CD8+ T cell epitope from chicken
                     ovalbumin"
     promoter        3452..3663
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             3676..3944
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4000..4665
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             4735..4788
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4789..5385
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5389..5977
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6051..6284
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6356..6490
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6496..6631
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6669..6685)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6693..6709)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6717..6747)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6762..6783)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7071..7659)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7833..8690)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8691..8795)
                     /label="AmpR promoter"
     primer_bind     9269..9285
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatggat caagccagat cagcattctc taacttgttt
     2341 ggtggggaac cattgtcata cacccggttt agccttgctc ggcaagtaga tggagataac
     2401 agtcatgtgg agatgaaact ggctgcagat gaagaagaaa atgccgacaa taacatgaag
     2461 gctagtgtca gaaaacccaa gaggtttaat ggaagactct gctttgcagc tattgcacta
     2521 gtcattttct tcttgattgg attcatgagt ggctacctgg gctattgtaa gcgtgtagaa
     2581 caaaaagagg agtgtgtgaa actggctgaa acggaggaga cagacaagtc agaaaccatg
     2641 gaaacagagg atgttgctgc agatcaagcc agagagctca tcaattcctg ggtagaaagt
     2701 cagacaaatg gaattatcag aaatgtcctt cagccaagct ccgtggattc tcaaactgca
     2761 atggttctgg ttaatgccat tgtcttcaaa ggactgtggg agaaaacatt taaggatgaa
     2821 gacacacaag caatgccttt cagagtgact gagcaagaaa gcaaacctgt gcagatgatg
     2881 taccagattg gtttatttag agtggcatca atggcttctg agaaaatgaa gatcctggag
     2941 cttccatttg ccagtgggac aatgagcatg ttggtgctgt tgcctgatga agtctcaggc
     3001 cttgagcagc ttgagagtat aatcaacttt gaaaaactga ctgaatggac cagttctaat
     3061 gttatggaag agaggaagat caaagtgtac ttacctcgca tgaagatgga ggaaaaatac
     3121 aacctcacat ctgtcttaat ggctatgggc attactgacg tgtttagctc ttcagccaat
     3181 ctgtctggca tctcctcagc agagagcctg aagatatctc aagctgtcca tgcagcacat
     3241 gcagaaatca atgaagcagg cagagaggtg gtagggtcag cagaggctgg agtggatgct
     3301 gcaagcgtct ctgaagaatt tagggctgac catccattcc tcttctgtat caagcacatc
     3361 gcaaccaacg ccgttctctt ctttggcaga tgtgtttccc cttaggatcc gcggccgcga
     3421 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     3481 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     3541 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     3601 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     3661 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     3721 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     3781 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     3841 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     3901 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     3961 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     4021 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     4081 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     4141 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     4201 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     4261 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     4321 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     4381 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     4441 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     4501 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     4561 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     4621 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     4681 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     4741 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     4801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     5401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5761 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5941 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     6001 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6061 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     6121 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6181 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6241 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6301 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6361 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6421 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6481 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6601 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     6661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7501 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8821 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8881 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9001 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9061 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9121 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9181 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9241 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
