LOCUS       .                       8362 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2302..4425
                     /label="large T antigen"
                     /note="large T antigen from SV40 (simian virus 40)"
     misc_feature    4441..5029
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5103..5336
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5408..5542
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5569..5704
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5737..5753)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5761..5777)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5785..5815)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5830..5851)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6139..6727)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6901..7758)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7759..7863)
                     /label="AmpR promoter"
     primer_bind     8337..8353
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggataaa gttttaaaca gagaggaatc tttgcagcta
     2341 atggaccttc taggtcttga aaggagtgcc tgggggaata ttcctctgat gagaaaggca
     2401 tatttaaaaa aatgcaagga gtttcatcct gataaaggag gagatgaaga aaaaatgaag
     2461 aaaatgaata ctctgtacaa gaaaatggaa gatggagtaa aatatgctca tcaacctgac
     2521 tttggaggct tctgggatgc aactgagatt ccaacctatg gaactgatga atgggagcag
     2581 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     2641 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     2701 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     2761 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     2821 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     2881 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     2941 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     3001 agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg tgggttaaag
     3061 gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg gaagcttgta
     3121 acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg gatgtacttg
     3181 gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca gcccagccac
     3241 tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag caaaaaccaa
     3301 aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt tgatagccta
     3361 caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga taggatggat
     3421 ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg agttgcttgg
     3481 ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa atgcatggtg
     3541 tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag tggtaaaact
     3601 acattagcag ctgctttgct tgaattatgt ggggggaaag ctttaaatgt taatttgccc
     3661 ttggacaggc tgaactttga gctaggagta gctattgacc agtttttagt agtttttgag
     3721 gatgtaaagg gcactggagg ggagtccaga gatttgcctt caggtcaggg aattaataac
     3781 ctggacaatt taagggatta tttggatggc agtgttaagg taaacttaga aaagaaacac
     3841 ctaaataaaa gaactcaaat atttccccct ggaatagtca ccatgaatga gtacagtgtg
     3901 cctaaaacac tgcaggccag atttgtaaaa caaatagatt ttaggcccaa agattattta
     3961 aagcattgcc tggaacgcag tgagtttttg ttagaaaaga gaataattca aagtggcatt
     4021 gctttgcttc ttatgttaat ttggtacaga cctgtggctg agtttgctca aagtattcag
     4081 agcagaattg tggagtggaa agagagattg gacaaagagt ttagtttgtc agtgtatcaa
     4141 aaaatgaagt ttaatgtggc tatgggaatt ggagttttag attggctaag aaacagtgat
     4201 gatgatgatg aagacagcca ggaaaatgct gataaaaatg aagatggtgg ggagaagaac
     4261 atggaagact cagggcatga aacaggcatt gattcacagt cccaaggctc atttcaggcc
     4321 cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg tagaggtttt
     4381 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataagg atccgtcgac
     4441 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4801 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4981 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     5041 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5101 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     5161 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5221 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5281 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5341 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5401 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5461 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5521 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5641 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5701 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     5761 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5821 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5881 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6541 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7741 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7861 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     7921 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     7981 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8041 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     8101 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8161 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     8221 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8281 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8341 aacgacggcc agtgccaagc tg
//
