LOCUS       .                       9920 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2290..4413
                     /label="large T antigen"
                     /note="large T antigen from SV40 (simian virus 40)"
     promoter        4463..4674
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             4687..4955
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4983..6005
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     misc_feature    6015..6603
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6677..6910
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6982..7116
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7122..7257
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7343..7373)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7388..7409)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7697..8285)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8459..9316)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9317..9421)
                     /label="AmpR promoter"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagccacca tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     2341 ggtcttgaaa ggagtgcctg ggggaatatt cctctgatga gaaaggcata tttaaaaaaa
     2401 tgcaaggagt ttcatcctga taaaggagga gatgaagaaa aaatgaagaa aatgaatact
     2461 ctgtacaaga aaatggaaga tggagtaaaa tatgctcatc aacctgactt tggaggcttc
     2521 tgggatgcaa ctgagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     2581 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     2641 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     2701 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     2761 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     2821 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     2881 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     2941 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     3001 agagatccat tttctgttat tgaggaaagt ttgccaggtg ggttaaagga gcatgatttt
     3061 aatccagaag aagcagagga aactaaacaa gtgtcctgga agcttgtaac agagtatgca
     3121 atggaaacaa aatgtgatga tgtgttgtta ttgcttggga tgtacttgga atttcagtac
     3181 agttttgaaa tgtgtttaaa atgtattaaa aaagaacagc ccagccacta taagtaccat
     3241 gaaaagcatt atgcaaatgc tgctatattt gctgacagca aaaaccaaaa aaccatatgc
     3301 caacaggctg ttgatactgt tttagctaaa aagcgggttg atagcctaca attaactaga
     3361 gaacaaatgt taacaaacag atttaatgat cttttggata ggatggatat aatgtttggt
     3421 tctacaggct ctgctgacat agaagaatgg atggctggag ttgcttggct acactgtttg
     3481 ttgcccaaaa tggattcagt ggtgtatgac tttttaaaat gcatggtgta caacattcct
     3541 aaaaaaagat actggctgtt taaaggacca attgatagtg gtaaaactac attagcagct
     3601 gctttgcttg aattatgtgg ggggaaagct ttaaatgtta atttgccctt ggacaggctg
     3661 aactttgagc taggagtagc tattgaccag tttttagtag tttttgagga tgtaaagggc
     3721 actggagggg agtccagaga tttgccttca ggtcagggaa ttaataacct ggacaattta
     3781 agggattatt tggatggcag tgttaaggta aacttagaaa agaaacacct aaataaaaga
     3841 actcaaatat ttccccctgg aatagtcacc atgaatgagt acagtgtgcc taaaacactg
     3901 caggccagat ttgtaaaaca aatagatttt aggcccaaag attatttaaa gcattgcctg
     3961 gaacgcagtg agtttttgtt agaaaagaga ataattcaaa gtggcattgc tttgcttctt
     4021 atgttaattt ggtacagacc tgtggctgag tttgctcaaa gtattcagag cagaattgtg
     4081 gagtggaaag agagattgga caaagagttt agtttgtcag tgtatcaaaa aatgaagttt
     4141 aatgtggcta tgggaattgg agttttagat tggctaagaa acagtgatga tgatgatgaa
     4201 gacagccagg aaaatgctga taaaaatgaa gatggtgggg agaagaacat ggaagactca
     4261 gggcatgaaa caggcattga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
     4321 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4381 aaacctccca cacctccccc tgaacctgaa acataaggat ccgcggccgc aaggatctgc
     4441 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4501 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4561 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4621 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     4681 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     4741 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     4801 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     4861 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     4921 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgtcg
     4981 acatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     5041 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     5101 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     5161 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     5221 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     5281 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     5341 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     5401 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     5461 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     5521 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     5581 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     5641 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     5701 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     5761 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     5821 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     5881 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     5941 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     6001 aggaataggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6421 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6601 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6661 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     6721 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6901 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6961 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7021 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7081 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7141 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7201 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7261 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7321 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7441 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7501 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8101 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8401 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9421 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9481 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9541 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9601 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     9661 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9721 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     9781 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9841 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9901 cgacggccag tgccaagctg
//
