LOCUS       V030805                10478 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030805
VERSION     V030805
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2311..2320
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2320..2349
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2350..4572
                     /label="Zbtb20(NM_019778)"
                     /note="Zbtb20(NM_019778)"
                     /gene="Zbtb20"
     promoter        4637..4848
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4861..5129
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5158..5167
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5188..5850
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5920..5973
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5974..6573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6574..7161
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7235..7468
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7540..7661
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7680..7815
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7853..7869)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7877..7893
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7901..7931)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7946..7967
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8255..8843)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9014..9874)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9875..9979)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10453..10469
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agcaaaagct catttctgaa
     2341 gaggacttga tgctagaacg gaagaaaccc aagacagctg aaaaccagaa ggcatctgag
     2401 gagaatgaga ttactcagcc gggcggatcc agcgccaagc cggcccttcc ctgcctgaac
     2461 tttgaagctg ttttgtctcc agccccagcc ctcatccact cgacacattc actgacaaac
     2521 tctcacgctc acaccgggtc atctgattgt gacatcagtt gcaaggggat gaccgagcgc
     2581 attcacagca tcaaccttca caacttcagc aattccgtgc tcgagaccct caacgagcag
     2641 cgcaaccgtg gccacttctg tgacgtgacg gttcgcatcc acgggagcat gctgcgcgca
     2701 catcgctgcg tgctggcagc cggcagcccc ttcttccaag acaagctgct gctgggctac
     2761 agcgacatcg aaatcccgtc ggtggtgtcc gtacaatcgg tgcaaaagct cattgacttc
     2821 atgtacagcg gtgtgctgag agtctcacag tcggaagctc tgcagatcct cacagccgcc
     2881 agcatcctgc agatcaaaac agtcatagat gagtgcactc gcatcgtgtc acagaacgtg
     2941 ggcgatgtgt tcccaggcat ccaggattct ggccaggaca caccaagagg cacaccagag
     3001 tcaggcacat ctggccagag cagtgacacg gaatcaggct acctgcagag ccacccacag
     3061 catagtgtgg accgaatcta ctccgcactc tacgcctgct ccatgcagaa tggcagcggc
     3121 gagcgctcct tctacagtgg tgcagtggtc agccaccacg aaacagctct cggcctgccc
     3181 cgtgaccacc acatggaaga ccctagctgg atcacacgca ttcatgagcg ctcccagcaa
     3241 atggagcgct acctgtccac cacccctgag accacgcact gccggaagca gccccggcct
     3301 gtgcgtatcc agaccctggt gggtaacatc cacatcaagc aggaaatgga agatgactat
     3361 gactactatg ggcagcaaag ggtgcagatc ctagaacgca atgaatccga ggagtgcaca
     3421 gaagacactg accaagcaga gggcactgag agcgagccca aaggtgaaag ctttgattct
     3481 ggggtcagct cctccatcgg caccgaacct gactcagtgg agcaacagtt tggggcagca
     3541 gccccaaggg acggtcaggc agaacccgcc caacctgagc aggcagcaga agccccagct
     3601 gagagcagtg cccagccaaa ccagctagaa ccaggtgcct cctctcctga gagaagcaac
     3661 gagtcagaga tggacaacac agtcatcact gtcagtaaca gctccgataa gggcgtccta
     3721 cagcagcctt cagtcaacac atccatcggg cagccattgc caagtaccca gctctattta
     3781 cgccagacag aaaccctcac cagcaacctg aggatgcctc tgaccttgac cagcaacaca
     3841 caggtcattg gcaccgctgg caacacctat ctgccagccc tcttcactac ccaacccgcg
     3901 ggcagtggcc ccaagccttt tctcttcagc ctgccgcagc ccctgacagg ccagcagacc
     3961 cagtttgtga cagtgtccca gcccggtctg tccaccttta ctgcacagct gccagcgcca
     4021 cagcccctgg cctcatctgc aggccacagc acagccagtg ggcaaggcga caaaaagcct
     4081 tatgagtgca ctctctgcaa caagactttc acagccaaac agaactacgt caagcacatg
     4141 ttcgtacata caggtgagaa gccccaccag tgcagcatct gctggcgctc cttctccttg
     4201 aaggattacc ttatcaagca catggtgacg cacaccggcg tgagagcgta ccagtgtagc
     4261 atctgcaaca agcgcttcac ccagaagagt tccctcaacg tgcacatgcg cctgcaccgc
     4321 ggggagaagt cctatgagtg ctacatctgc aaaaagaagt tctcccacaa gaccctgctg
     4381 gagcgacacg tggccctgca cagtgccagc aacgggaccc ctccggcagg cacgccccca
     4441 ggtgcccgcg cgggtccgcc aggcgtggtg gcctgcacag aggggaccac ttacgtctgc
     4501 tccgtctgcc cagcaaagtt tgaccaaatc gagcagttca acgaccacat gaggatgcat
     4561 gtgtctgacg gataaggatc cctgcgatat ccagcgcggc cgcgaaggat ctgcgatcgc
     4621 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4681 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4741 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4801 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     4861 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     4921 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     4981 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     5041 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     5101 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     5161 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     5221 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     5281 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     5341 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     5401 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     5461 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     5521 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     5581 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     5641 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     5701 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     5761 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     5821 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     5881 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     5941 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     6001 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6061 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6121 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6181 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6241 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6301 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6361 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6421 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6481 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6541 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     6601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6961 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7081 ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc
     7141 tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     7201 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7261 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7441 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7501 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7561 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7621 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7681 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7741 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7801 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc
     7861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8041 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10021 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10081 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10141 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10201 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10261 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
    10321 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10381 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10441 agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
