LOCUS       V047372                10904 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047372
VERSION     V047372
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5061
                     /label="ZMIZ2(NM_031449)"
                     /note="ZMIZ2(NM_031449)"
                     /gene="ZMIZ2"
     CDS             5068..5121
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5122..5832
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5874..6085
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6098..6366
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6393..6992
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6999..7587
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7661..7894
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7966..8087
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8106..8241
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8279..8295)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8303..8319
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8327..8357)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8372..8393
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8681..9269)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9440..10300)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10301..10405)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10879..10895
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaactcc atgaacccca tgaaacctgc cctgccccct
     2341 gcgccacacg gtgatggttc attcgcatat gagtctgtgc cttggcaaca aagcgccact
     2401 cagccggctg gatcgctgtc tgtggtcact actgtgtggg gagttggcaa cgcgacacag
     2461 agccaggttt tggggaaccc catgggccct gcagggagtc cctctggcag ctccatgatg
     2521 cctggtgtgg cagggggcag ctccgccttg acctccccac agtgcctggg acagcaggcg
     2581 tttgctgaag gcggcgccaa caagggctac gtgcagcaag gcgtgtacag ccgcgggggc
     2641 taccctgggg cccccggctt caccaccggg tatgcaggcg gcccgggggg cctgggcctc
     2701 ccctcacatg ctgcaagacc ctccactgac ttcacgcaag cggcagctgc tgcagctgtg
     2761 gctgctgcgg cagccactgc caccgccaca gccacagcca ccgtggctgc tctccaggag
     2821 aagcagagcc aggagctgag ccagtatgga gcgatggggg ccggacagtc ttttaacagc
     2881 cagtttctgc agcatggagg tccccggggg cctagtgtcc ccgctggcat gaaccctact
     2941 ggcataggag gggtaatggg cccctctggc ctctccccct tggctatgaa ccccacccgg
     3001 gcagcaggaa tgacaccctt gtatgcaggg cagcgtctgc cccaacatgg gtatcctggg
     3061 cctccccagg cccagccact gccccgacag ggggtcaaga gaacctactc tgaggtgtat
     3121 ccagggcagc agtatctgca aggaggccag tatgcaccca gcaccgccca gtttgcgccc
     3181 agccctgggc agccccctgc cccctcccct tcctaccctg ggcacaggct gcccctgcag
     3241 cagggcatga cccagtccct gtccgtgcct ggccccacgg gactgcatta taagcccaca
     3301 gagcagttca acgggcaggg cgccagcttc aacgggggca gcgtcagcta cagccaacct
     3361 ggcctgagtg ggcctacccg ttccatcccg ggctatccca gttccccact gccagggaac
     3421 cccacgccac ccatgacccc aagcagcagc gtcccttaca tgtcaccaaa ccaagaggtc
     3481 aagtctccct tcttgcctga tctcaagccc aacctcaact ccttgcactc atcgccctct
     3541 ggaagcgggc cttgtgacga gttgcggctg accttccctg tgcgcgatgg ggtggtcctg
     3601 gagcccttcc gcctgcagca caacctggct gtaagcaacc atgtcttcca gctgcgagac
     3661 tcagtctaca agaccctgat aatgaggcct gacctggagc tgcaattcaa gtgctaccac
     3721 cacgaggacc ggcagatgaa caccaactgg ccagcctcgg tgcaggtcag cgtcaatgcc
     3781 acgccgctca ccatcgagcg tggcgacaac aagacctcgc acaagccact ctacctgaag
     3841 catgtgtgcc agccaggccg caacaccatc cagatcaccg tcaccgcctg ctgctgctcc
     3901 cacctcttcg tgctgcagct agtgcaccgc ccatccgtcc gctcggtgct gcagggcctc
     3961 ctcaaaaagc gcctcctgcc tgctgagcac tgcatcacca agataaagcg gaacttcagc
     4021 agcggcacca tccctggcac ccctgggccc aacggagagg acggggtgga gcagacagct
     4081 atcaaggtgt ccctgaagtg ccccatcacc ttccgcagga tccagctccc tgcccgaggt
     4141 catgactgtc gccacataca gtgctttgac ctggagtcgt acctgcagct caactgtgag
     4201 cgggggactt ggaggtgtcc tgtgtgcaac aagacagctt tgctggaggg cctggaggtg
     4261 gaccagtaca tgctgggcat cctgatttac attcagaact ctgactatga ggagatcacc
     4321 atcgacccca cgtgcagctg gaagccagtg cccgtgaagc ctgacatgca catcaaggag
     4381 gagccggatg ggccagcact gaagcgctgc cgcaccgtga gccccgccca cgtgctcatg
     4441 cccagcgtga tggagatgat cgccgccctg ggccccggcg ctgccccctt tgcccccctg
     4501 cagcccccct cagtccctgc ccccagcgac taccctggcc agggttccag cttcctgggg
     4561 cctggaactt tccctgagtc cttcccaccc accacgccca gcaccccaac ccttgctgag
     4621 ttcaccccgg gaccaccccc catctcctac cagtctgaca ttcccagcag cctcctgact
     4681 tcagagaagt ctaccgcctg cctcccaagc cagatggcac cagcaggtca cctggacccc
     4741 actcacaatc ctgggacacc aggactacac acctccaacc ttggggcccc tccaggtccc
     4801 cagctgcacc attcaaaccc tcccccagcg tcccggcagt ccttgggcca agcgagctta
     4861 ggacctacgg gtgaactggc cttcagtcct gccacaggcg tgatggggcc ccccagcatg
     4921 tctggagccg gggaggcccc agaaccagct ctggacctgc tcccggaact gaccaaccct
     4981 gatgagctac tgtcctactt gggcccaccc gacctcccta cgaacaacaa tgacgacctg
     5041 ctttctctgt ttgagaacaa cggatccgag ggcagaggaa gtcttctaac atgcggtgac
     5101 gtggaggaga atcccggccc tatggtgagc aagggcgagg aggataacat ggccatcatc
     5161 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     5221 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     5281 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     5341 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     5401 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     5461 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     5521 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     5581 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     5641 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     5701 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5761 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5821 ctgtacaagt aggcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     5881 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     5941 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     6001 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     6061 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     6121 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     6181 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     6241 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     6301 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     6361 cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc
     6421 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     6481 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     6541 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6601 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6661 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6721 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6781 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6841 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6901 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6961 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa
     7021 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7381 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7561 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7621 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7681 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7741 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7801 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7861 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7921 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7981 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8041 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8101 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8161 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8221 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     8281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9121 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10441 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10501 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10561 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10621 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10681 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10741 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10801 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10861 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
