LOCUS       V047640                10788 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047640
VERSION     V047640
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4807
                     /label="ZHX2(NM_001362797)"
                     /note="ZHX2(NM_001362797)"
                     /gene="ZHX2"
     CDS             4814..4903
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4947..5158
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5171..5439
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5468..5477
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5498..6160
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6230..6283
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6284..6883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6884..7471
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7545..7778
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7850..7971
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7990..8125
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8163..8179)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8187..8203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8211..8241)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8256..8277
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8565..9153)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9324..10184)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10185..10289)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10763..10779
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg ctagcaaacg aaaatctaca actccatgca tggttcggac
     2341 atcacaagta gtagaacaag atgtgcccga ggaagtagac agggccaaag agaaaggaat
     2401 cggcacacca cagcctgacg tggccaagga cagttgggca gcagaacttg aaaactcttc
     2461 caaagaaaac gaagtgatag aggtgaaatc tatgggggaa agccagtcca aaaaactcca
     2521 aggtggttat gagtgcaaat actgccccta ctccacgcaa aacctgaacg agttcacgga
     2581 gcatgtcgac atgcagcatc ccaacgtgat tctcaacccc ctctacgtgt gtgcagaatg
     2641 taacttcaca accaaaaagt acgactccct atccgaccac aactccaagt tccatcccgg
     2701 ggaggccaac ttcaagctga agttaattaa acgcaataat caaactgtct tggaacagtc
     2761 catcgaaacc accaaccatg tcgtgtccat caccaccagt ggccctggaa ctggtgacag
     2821 tgattctggg atctcggtga gtaaaacccc catcatgaag cctggaaaac caaaagcgga
     2881 tgccaagaag gtgcccaaga agcccgagga gatcaccccc gagaaccacg tggaagggac
     2941 cgcccgcctg gtgacagaca cagctgagat cctctcgaga ctcggcgggg tggagctcct
     3001 ccaagacaca ttaggacacg tcatgccttc tgtacagctg ccaccaaata tcaaccttgt
     3061 gcccaaggtc cctgtcccac taaatactac caaatacaac tctgccctgg atacaaatgc
     3121 cacgatgatc aactctttca acaagtttcc ttacccgacc caggctgagt tgtcctggct
     3181 gacagctgcc tccaaacacc cagaggagca catcagaatc tggtttgcca cccagcgctt
     3241 aaagcatggc atcagctggt ccccagaaga ggtggaggag gcccggaaga agatgttcaa
     3301 cggcaccatc cagtcagtac ccccgaccat cactgtgctg cccgcccagt tggcccccac
     3361 aaaggtgacg cagcccatcc tccagacggc tctaccgtgc cagatcctcg gccagactag
     3421 cctggtgctg actcaggtga ccagcgggtc aacaaccgtc tcttgctccc ccatcacact
     3481 tgccgtggca ggagtcacca accatggcca gaagagaccc ttggtgactc cccaagctgc
     3541 ccccgaaccc aagcgtccac acatcgctca ggtgccagag cccccaccca aggtggccaa
     3601 ccccccgctc acaccagcca gtgaccgcaa gaagacaaag gagcagatag cacatctcaa
     3661 ggccagcttt ctccagagcc agttccctga cgatgccgag gtttaccggc tcatcgaggt
     3721 gactggcctt gccaggagcg agatcaagaa gtggttcagt gaccaccgat atcggtgtca
     3781 aaggggcatc gtccacatca ccagcgaatc ccttgccaaa gaccagttgg ccatcgcggc
     3841 ctcccgacac ggtcgcacgt atcatgcgta cccagacttt gccccccaga agttcaaaga
     3901 gaaaacacag ggtcaggtta aaatcttgga agacagcttt ttgaaaagtt cttttcctac
     3961 ccaagcagaa ctggatcggc taagggtgga gaccaagctg agcaggagag agatcgactc
     4021 ctggttctcg gagaggcgga agcttcgaga cagcatggaa caagctgtct tggattccat
     4081 ggggtctggc aaaaaaggcc aagatgtggg agcccccaat ggtgctctgt ctcgactcga
     4141 ccagctctcc ggtgcccagt taacaagttc tctgcccagc ccttcgccag caattgcaaa
     4201 aagtcaagaa caggttcatc tcctgaggag cacgtttgca agaacccagt ggcctactcc
     4261 ccaggagtac gaccagttag cggccaagac tggcctggtc cgaactgaga ttgtgcgttg
     4321 gttcaaggag aacagatgct tgctgaaaac gggaaccgtg aagtggatgg agcagtacca
     4381 gcaccagccc atggcagatg atcacggcta cgatgccgta gcaaggaaag caacaaaacc
     4441 catggccgag agcccaaaga acgggggtga tgtggttcca caatattaca aggaccccaa
     4501 aaagctctgc gaagaggact tggagaagtt ggtgaccagg gtaaaagtag gcagcgagcc
     4561 agcaaaagac tgtttgccag caaagccctc agaggccacc tcagaccggt cagagggcag
     4621 cagccgggac ggccagggta gcgacgagaa cgaggagtcg agcgttgtgg attacgtgga
     4681 ggtgacggtc ggggaggagg atgcgatctc agatagatca gatagctgga gtcaggctgc
     4741 ggcagaaggt gtgtcggaac tggctgaatc agactccgac tgcgtccctg cagaggctgg
     4801 ccaggccgga tccgaacaaa aactaatatc agaggaagat ttggaacaga aactaatcag
     4861 tgaagaagat ttagagcaaa agttaatttc cgaggaggac ttatagcggc cgcgaaggat
     4921 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4981 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5041 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5101 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5161 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5221 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5281 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5341 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5401 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5461 gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
     5521 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
     5581 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
     5641 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
     5701 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
     5761 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
     5821 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
     5881 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
     5941 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
     6001 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
     6061 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
     6121 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc
     6181 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg
     6241 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc
     6301 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6361 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6481 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta
     6901 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6961 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7021 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7081 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7141 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7261 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7321 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7381 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac
     7441 gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7561 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7801 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7981 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8041 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8101 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     8161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9001 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10321 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10381 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    10441 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    10501 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    10561 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    10621 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10681 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    10741 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
