LOCUS V044499 10624 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044499 VERSION V044499 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4650 /label="ZDHHC8(NM_001185024)" /note="ZDHHC8(NM_001185024)" /gene="ZDHHC8" CDS 4657..4722 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4766..4977 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4990..5258 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5287..5296 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5317..5979 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6049..6102 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6103..6702 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6703..7291 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7365..7598 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7670..7791 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7831..7966 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7999..8015) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8023..8039 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8047..8077) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8092..8113 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8401..8989) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9160..10020) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10021..10125) /gene="bla" /label="AmpR promoter" primer_bind 10599..10615 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cccgcagccc cgggacgcgc 2341 ctcaaacccg ccaagtacat cccggtggcc acggccgccg cgctgctggt cggctccagc 2401 accctcttct tcgtgttcac gtgcccgtgg ttgacacgag ctgtgtcccc agctgttccc 2461 gtctacaatg gcatcatctt cctctttgtc ctggccaact tcagcatggc cactttcatg 2521 gaccctggtg ttttcccccg agcggatgag gatgaggaca aggaggacga cttccgggct 2581 ccgctgtaca agaacgtgga tgtgcgaggt atccaggtcc gcatgaagtg gtgtgccacg 2641 tgccacttct accgcccgcc gcgctgctcc cactgcagcg tctgtgacaa ctgtgtagag 2701 gactttgacc accactgccc ctgggtcaac aactgcatcg ggcgtcgaaa ctatcgctac 2761 ttcttcctgt tcctgctgtc actcagtgca cacatggtgg gcgtcgtggc cttcggcctg 2821 gtctacgtgc tgaaccacgc tgaggggctg ggagccgcgc acaccaccat caccatggct 2881 gtcatgtgtg tggccggcct cttcttcatc cctgtcattg gcctcactgg cttccatgtg 2941 gtgctggtca ctcgggggcg caccaccaac gagcaggtga ctgggaagtt ccgcgggggt 3001 gtgaaccctt tcacccgagg ctgctgtggg aatgtggagc acgtgctgtg tagccccctg 3061 gcgccccggt acgtggtgga gccaccccgg ctgccgctcg cggtgagttt gaagccgcct 3121 ttccttaggc ctgaactcct ggaccgagct gcaccgctca aggtcaagct tagtgacaac 3181 gggctgaagg ctggcctggg ccgtagcaag tccaagggca gcctggaccg gctggatgag 3241 aagccactgg acttggggcc accactgccc cccaagatag aggctggcac gttcagcagt 3301 gacctgcaga ccccgcgccc aggcagtgct gagagtgccc tgtcggtgca gaggaccagc 3361 cccccgacac ctgccatgta caagtttagg ccggctttcc ccacgggtcc caaggtgccc 3421 ttctgtggac caggcgagca ggttccaggc cctgattccc tgaccctggg ggacgacagc 3481 atccgtagcc tggactttgt gtccgagccg agcctggacc tccctgacta tgggccaggg 3541 ggcctgcatg cagcctaccc gccatcccca ccgctcagcg cctctgatgc cttctcgggc 3601 gctttgcgct ccctgagcct caaggcctcg agccggcggg gcggggatca tgtggccctg 3661 cagcccctgc gctctgaggg ggggcccccc acgccccacc gtagcatttt tgccccccat 3721 gcactgccca accgcaacgg cagcctgtcc tatgacagcc tgctcaatcc tggctcgcct 3781 ggtggccacg cctgccctgc ccacccagca gttggcgtgg ccggatacca ctcaccctac 3841 ctgcatcctg gggcaacggg cgacccgcca cggcccctac cccgcagctt cagccccgtg 3901 ctgggccccc gcccccggga gccctcgcct gtgcgctacg acaacctgtc caggaccatc 3961 atggcatcca tccaggagcg caaggacagg gaggagcgtg agcgcctgct gcgctcccag 4021 gccgactcac tcttcggcga ctcaggcgtc tatgacgctc ccagctccta cagcctgcag 4081 caggccagtg tgctgtccga gggcccccga ggtcccgcgc tgcgctatgg ctccagagac 4141 gaccttgtgg ctgggcccgg cttcggtggc gcccgcaacc ctgccctgca gacgtcactg 4201 tcctcgctgt ccagctccgt gagccgtgca ccgcggacgt cgtcctcctc cctgcaggct 4261 gatcaggcca gcagcaacgc cccggggccc cggcccagca gtggctcaca caggtcacct 4321 gcacgccagg gcctgccctc cccgcccggc actccccact caccatccta cgcgggcccc 4381 aaagctgtcg ccttcatcca cacggacctc ccagagccac cgccctcgct gaccgtgcag 4441 agggggcgga ttggcacctg cacccgtgga tgggggcggc gtggccagcc ttgggtgcct 4501 cctgggctgc acctgtgcca ccttggccgc ccggaggacc gcccaccact gcgggccccc 4561 tggagccagg ccgccggggc acccccacgc ggggccatgt gccgcctgca cttggctgcc 4621 tccagtcttt tccccagcct ctcggggccc ggatccgact acaaagacca tgacggtgat 4681 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc 4741 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4801 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4861 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4921 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4981 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 5041 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 5101 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 5161 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 5221 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 5281 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 5341 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5401 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5461 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5521 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5581 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5641 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5701 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5761 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5821 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5881 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5941 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 6001 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 6061 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 6121 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6181 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 6241 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6301 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6361 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6421 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6481 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6541 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6601 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6661 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 6721 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 6781 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 6841 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6901 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6961 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 7021 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 7081 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg 7141 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 7201 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 7261 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7321 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7381 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7441 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7501 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7561 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7621 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7801 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 7861 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 7921 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7981 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 8041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 8101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 8161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 8221 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 8281 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8581 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8821 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8941 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 9001 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 9061 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 9121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 9181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 9241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 9301 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 10021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 10081 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 10141 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 10201 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 10261 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 10321 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 10381 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10441 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10501 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10561 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 10621 gctg //