LOCUS       V036381                 9553 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036381
VERSION     V036381
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4446
                     /label="ZDHHC5(NM_015457)"
                     /note="ZDHHC5(NM_015457)"
                     /gene="ZDHHC5"
     CDS             4453..4479
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4523..4734
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4747..5015
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5042..5641
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5648..6236
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6310..6543
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6615..6736
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6755..6890
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6928..6944)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6952..6968
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6976..7006)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7021..7042
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7330..7918)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8089..8949)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8950..9054)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9528..9544
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcccgca gagtctggaa agagattcaa acccagcaag
     2341 tatgtcccgg tctctgcagc cgccatcttc ctagtgggag ctacgacact cttctttgcc
     2401 tttacgtgtc caggactaag cctgtatgtg tcacctgcag tgcccatcta caatgcaatt
     2461 atgtttctct ttgtgttggc caacttcagc atggccacct tcatggaccc agggattttc
     2521 cctcgagctg aggaggatga ggacaaggaa gatgatttcc gagctcccct ttacaaaaca
     2581 gtggagataa agggcatcca ggtgcgcatg aaatggtgtg ccacctgccg cttttaccgt
     2641 ccccctcgat gttcccactg cagtgtctgt gacaactgtg tggaggaatt tgatcatcac
     2701 tgcccctggg tgaataactg tattggtcgc cggaactacc gttatttttt ccttttcctc
     2761 ctttccctga cagcccacat tatgggtgtg tttggctttg gcctccttta tgtcctctac
     2821 cacatagagg aactctcagg ggtccgcacg gctgtcacaa tggcagtaat gtgtgtggct
     2881 ggcttattct tcatccctgt agctggcctc acgggatttc acgtggttct ggtggccagg
     2941 ggacgcacaa ccaatgaaca ggttacgggt aaattccggg gaggtgtgaa ccccttcacc
     3001 aatggctgct gtaacaatgt cagccgtgtt ctctgcagtt ctccagcacc caggtatttg
     3061 gggagaccaa agaaagagaa gacaattgta atcagacctc ccttccttcg accagaagtt
     3121 tcagatgggc agataactgt gaagatcatg gataatggca tccagggaga gctgaggaga
     3181 acaaagtcta agggaagcct ggagataaca gagagccagt ctgcagatgc tgaacctcca
     3241 cctcctccta agccagacct gagccgttac acagggttgc gaacacacct cggcttggct
     3301 actaatgagg atagtagctt attggccaag gacagccccc cgacacctac catgtacaag
     3361 tatcggccgg gttacagtag cagcagtacg tcagctgcca tgccgcattc ctccagcgcc
     3421 aagttgagtc gtggggacag cttgaaggag ccaacctcaa ttgcagagag cagccgtcac
     3481 cccagctacc gctcagagcc cagcttggaa ccagagagct tccgttctcc tacctttggc
     3541 aaaagttttc acttcgatcc actatccagt ggctcacgct cctccagcct caagtcagcc
     3601 cagggcacag gctttgagct gggccagttg caatccattc gttcagaggg caccacctcc
     3661 acctcctata agagcctggc caaccagaca cgcaatggaa gcctatctta tgacagcttg
     3721 ctcacacctt cagacagccc tgattttgag tcagtgcagg cagggcctga gccagaccca
     3781 cctttaggct atacctctcc cttcctgtca gccaggctgg cccagcaacg ggaagctgag
     3841 aggcacccac gtttggtgcc aactggccca acacaccgag agccctcacc agtccgttac
     3901 gacaatctgt cgcgccacat tgtggcctct ctccaggaac gagagaagtt gctgcgccag
     3961 tcacccccac tcccgggccg tgaggaagaa ccaggcttgg gggactcagg cattcagtca
     4021 acaccaggct cgggccatgc ccctcgtact agttcctcct cagatgattc aaagagatca
     4081 cctttgggca agactccact gggacgccca gctgtccccc gttttggcaa gccagatggg
     4141 ctaaggggcc ggggagtagg gtcccctgaa ccaggcccaa cagccccata cctgggccga
     4201 tcgatgtctt acagcagcca aaaagcccaa cctggtgtct ctgagacaga agaagtggcc
     4261 ttgcagccat tactgacacc caaagatgaa gtacagctga agaccaccta cagcaaatcc
     4321 aacgggcagc ccaagagctt aggctcagcc tcccctggcc caggccagcc acctctcagt
     4381 agccccacga ggggaggagt caagaaggtg tcaggggttg gtggtaccac ctatgagatt
     4441 tcggtgggat cctacccata cgacgtacca gattacgctt agcggccgcg aaggatctgc
     4501 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4561 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4621 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4681 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     4741 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     4801 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     4861 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     4921 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     4981 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5041 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     5101 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     5161 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5221 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5281 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5341 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5401 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5461 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5521 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5581 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5641 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5701 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5761 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5821 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5881 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5941 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6001 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6061 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6121 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6181 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6241 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6301 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     6361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6541 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6601 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6661 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6721 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     6781 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6841 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     6901 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7081 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7141 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7741 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9061 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9121 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9181 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9241 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9301 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9361 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9421 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9481 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9541 cagtgccaag ctg
//
