LOCUS       V043356                10017 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043356
VERSION     V043356
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4212
                     /label="ZDHHC17(NM_015336)"
                     /note="ZDHHC17(NM_015336)"
                     /gene="ZDHHC17"
     CDS             4219..4245
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4249..4275
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4279..4305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4370..4581
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4594..4862
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4891..4900
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4921..5583
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5653..5706
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5707..6105
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6112..6700
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6774..7007
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7079..7200
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7219..7354
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7392..7408)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7416..7432
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7440..7470)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7485..7506
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7794..8382)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8553..9413)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9414..9518)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9992..10008
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agcgggagga gggatttaac
     2341 accaagatgg cggacggccc ggatgagtac gataccgaag cgggctgtgt gccccttctc
     2401 cacccagagg aaatcaaacc ccaaagccat tataaccatg gatatggtga acctcttgga
     2461 cggaaaactc atattgatga ttacagcaca tgggacatag tcaaggctac acaatatgga
     2521 atatatgaac gctgtcgaga attggtggaa gcaggttatg atgtacggca accggacaaa
     2581 gaaaatgtta ccctcctcca ttgggctgcc atcaataaca gaatagattt agtcaaatac
     2641 tatatttcga aaggtgctat tgtggatcaa cttggagggg acctgaattc aactccattg
     2701 cactgggcca caagacaagg ccatctatcc atggttgtgc aactaatgaa atatggtgca
     2761 gatccttcat taattgatgg agaaggatgt agctgtattc atctggctgc tcagttcgga
     2821 catacctcaa ttgttgctta tctcatagca aaaggacagg atgtagatat gatggatcag
     2881 aatggaatga cgcctttaat gtgggcagca tatagaacac atagtgtgga tccaactaga
     2941 ttgcttttaa cattcaatgt ttcagttaac cttggtgaca agtatcacaa aaacactgct
     3001 ctgcattggg cagtgctagc agggaatacc acagtcatta gccttcttct ggaagctgga
     3061 gctaatgttg atgcccagaa tatcaagggc gaatcagcgc ttgatttggc aaaacagaga
     3121 aaaaatgtgt ggatgatcaa ccacttacaa gaggcaaggc aagcaaaagg atatgacaat
     3181 ccgtccttcc ttagaaagct gaaagctgat aaggaatttc ggcagaaagt aatgttagga
     3241 actcctttcc tagttatttg gctggttggg tttatagcag acctaaatat tgattcttgg
     3301 ctcattaaag ggctaatgta tggtggtgtt tgggctacag tacagtttct ttcaaaatcc
     3361 tttttcgatc attcaatgca tagtgcattg ccccttggga tatatttggc aaccaaattc
     3421 tggatgtatg tgacgtggtt cttctggttt tggaatgatc tcaacttttt atttatccat
     3481 cttccattcc ttgccaatag tgttgcactt ttctacaatt ttggaaaatc ttggaaatca
     3541 gatccaggga ttattaaagc aacagaagag caaaagaaaa agacaatagt tgaacttgca
     3601 gagacaggaa gtctggacct cagtatattc tgcagtacct gtttgatacg aaaaccggtg
     3661 aggtccaaac attgtggtgt gtgcaaccgc tgtatagcaa aatttgatca tcattgccca
     3721 tgggtgggta actgtgtagg tgcaggcaac catagatatt ttatgggcta cctattcttc
     3781 ttgcttttta tgatctgctg gatgatttat ggttgtatat cttactgggg actccactgt
     3841 gagaccactt acaccaagga tggattttgg acatacatta ctcagattgc cacgtgttca
     3901 ccttggatgt tttggatgtt cctgaacagt gttttccact tcatgtgggt ggctgtatta
     3961 ctcatgtgtc agatgtacca gatatcatgt ttaggtatta ctacaaatga aagaatgaat
     4021 gccaggagat acaagcactt taaagtcaca acaacgtcta ttgaaagccc attcaaccat
     4081 ggatgtgtaa gaaatattat agacttcttt gaatttcgat gctgtggcct ctttcgtcct
     4141 gttatcgtgg actggaccag gcagtataca atagaatatg accaaatatc aggatctggg
     4201 taccagctgg tgctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     4261 gtacctgatt atgctagcta tccttatgat gtcccagact acgcataggg atccctgcga
     4321 tatccagcgc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4381 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4441 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4501 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4561 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4621 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4681 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4741 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4801 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4861 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     4921 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4981 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5041 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5101 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5161 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5221 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5281 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5341 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5401 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5461 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5521 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5581 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5641 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5701 ggccctatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     5761 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     5821 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     5881 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     5941 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     6001 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     6061 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagtcga caatcaacct
     6121 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6481 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6661 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     6721 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6781 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt
     6841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7021 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7201 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     7261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7321 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat
     7381 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7441 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7561 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7621 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7741 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7801 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7861 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7921 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7981 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8041 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8101 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8161 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8221 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8281 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8341 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8401 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8461 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8521 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8581 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8641 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8701 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8761 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8821 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8881 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8941 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9001 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9061 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9541 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9601 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     9661 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     9721 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     9781 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     9841 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     9901 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     9961 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
