LOCUS       V040859                11173 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040859
VERSION     V040859
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4182
                     /label="ZDHHC13(NM_019028)"
                     /note="ZDHHC13(NM_019028)"
                     /gene="ZDHHC13"
     CDS             4213..5169
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     CDS             5206..5271
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5315..5526
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5539..5807
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5836..5845
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5866..6528
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6598..6651
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6652..7251
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7252..7840
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7914..8147
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8219..8340
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8380..8515
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8548..8564)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8572..8588
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8596..8626)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8641..8662
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8950..9538)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9709..10569)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10570..10674)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11148..11164
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggggccggg gctgggctcg
     2341 cagtgcagga atcacagcca tggcccccac cctccaggat ttggtcgata tggcatctgt
     2401 gcacatgaaa acaaagaact tgccaatgca agagaagctc ttcctcttat agaggactct
     2461 agtaactgtg acattgtcaa agctactcaa tacggaattt ttgaacgatg taaagagttg
     2521 gtagaagcag gatatgatgt caggcaacca gataaagaaa atgtgtcgct tcttcattgg
     2581 gctgctatta acaacagact ggatcttgta aagttttata tttcaaaagg tgctgttgta
     2641 gatcagttgg gtggagattt aaattcaact cctcttcact gggccatccg acaaggacat
     2701 ttacctatgg tcatattatt actccagcat ggtgcagacc ccactcttat tgatggagag
     2761 ggattcagca gcatccacct ggcagtattg tttcaacaca tgcctattat agcatatctc
     2821 atctcaaagg gacagagtgt gaatatgaca gatgtaaatg ggcagacacc tctcatgtta
     2881 tcagctcaca aagtaattgg gccagaacca actggatttc ttttaaagtt taatccttct
     2941 ctcaatgtgg ttgataaaat acaccaaaac actccacttc actgggcagt tgcagcagga
     3001 aatgttaatg cagttgataa gcttttggaa gctggttcta gcctggatat ccagaatgtt
     3061 aagggagaaa cacctcttga tatggctcta caaaacaaaa atcagctcat tattcatatg
     3121 ctaaaaacag aagccaaaat gagagccaac caaaagttca gactttggag gtggctgcag
     3181 aaatgcgagc tcttcctgct gctgatgctt tctgtgatta ccatgtgggc tattggatac
     3241 atattggact tcaattcaga ttcttggctt ttaaaaggat gtcttctagt aacactgttt
     3301 tttctgacat ctttgtttcc aaggttcttg gttgggtata agaaccttgt atacttacca
     3361 acagcctttc tgctaagttc tgttttttgg atatttatga cttggttcat cttatttttt
     3421 cctgatttag caggagcccc tttctatttc agtttcattt tcagcatagt agcctttcta
     3481 tactttttct ataagacttg ggcaactgat ccaggcttca ctaaggcttc tgaagaagaa
     3541 aagaaagtga atatcatcac ccttgcagaa actggctctc tggacttcag aacattttgt
     3601 acatcatgtc ttataaggaa gccattaagg tcactccact gccatgtatg caactgctgt
     3661 gtggctcgat atgatcaaca ctgcctgtgg actggacggt gcataggttt tggcaaccat
     3721 cactattaca tattcttctt gtttttcctt tccatggtat gtggctggat tatatatgga
     3781 tctttcatct atttgtccag tcattgtgcc acaacattca aagaagatgg attatggact
     3841 tacctcaatc agattgtggc ctgttcccct tgggttttat atatcttgat gctagcaact
     3901 ttccatttct catggtcaac atttttatta ttaaatcaac tctttcagat tgcctttctg
     3961 ggcctgacct cccatgagag aatcagcctg cagaagcaga gcaagcatat gaaacagacg
     4021 ttgtccctca ggaagacacc atacaatctt ggattcatgc agaacctggc agatttcttt
     4081 cagtgtggct gctttggctt ggtgaagccc tgtgtggtag attggacatc acagtacacc
     4141 atggtctttc acccagccag ggagaaggtt cttcgctcag taggtggagg cggatcagga
     4201 ggcggtggat ctaaagacaa tactgtgcct ctgaagctga tcgctctcct ggctaatggc
     4261 gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc cgctatcaac
     4321 aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc
     4381 tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg acagctggac
     4441 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     4501 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     4561 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     4621 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     4681 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     4741 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct
     4801 gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg
     4861 gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg
     4921 gccgctacgc tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg
     4981 gctccatatc tgccacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg
     5041 atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg
     5101 ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct
     5161 gccgaaaagg gtggaggcgg atcaggaggc ggtggatctg gatccgacta caaagaccat
     5221 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5281 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5341 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5401 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5461 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5521 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5581 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5641 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5701 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5761 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5821 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     5881 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     5941 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6001 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6061 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6121 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6181 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6241 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6301 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6361 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6421 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     6481 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     6541 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     6601 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     6661 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6721 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6781 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6841 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6901 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6961 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7021 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7081 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7141 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7201 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     7261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7801 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     7861 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7921 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8161 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8221 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8281 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8341 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8461 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8521 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9361 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10681 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10741 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10801 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10861 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10921 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10981 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11041 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11101 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11161 cagtgccaag ctg
//
